Sequences from confirmation amplicon


Confirmation sequences for line 783B09 and hit At5g42820 are shown.
Line ID 783B09
Locus At5g42820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17171699
Primers used for PCR CS48 - 8474, show primer details
   
Sequence Name 28-K107428-0022-783-B09C-CS48
GenBank Accession LN498874 [GenBank]
Sequencing primer gene specific primer, CS48: 5'-TCGTAGAAATCCTCAAAGTGGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K107428-0022-783-B09C-CS48
CCTGGAGTTATCATGTCAGGTCTCTGGTACATGTTCGAGAGGAGAAGCGTCGGCGAGATG
GTGGGACGGTTATGAAGCCGTGAGCACCGGTCACCGTGACGACAAGCACCAATCTTAAAG
TAAAACGGACAATTCACTCTATCCTTTTCAGTACCAAAGATTGAAGCTAAATGCTCTGCC
ATTCTCACCTAAACATAACAAAAATCAAATTCAACTAAACACAAATTGTTCTTCTACGCT
TATATGATAAATCAAATCACTACAAGAACGATTCAGAATCAGAGACGAGAACCAAATCTA
AGCTTATAAACGAGAGTTAACACAAAATCAAAGAGAATCAAATCAATGAACGAATCAGAA
TCATGAATCGTTGTCAAATCTAAAACAGATCGAGACGTTTCTAGTCATCGAGTGGAGATG
AGAATCCGAAACGGAGAGAGAGATTGATTAGAGGAAGAAGAATACCGAAGAGGATTTTCT
TTTAGTGGAAGAGAAGCTATCGCCGACGAGGAGGTGCCGTTGCCGATCACGATCTAGGGT
TTAGGTTTATTTGATTAGCGACTCGTGTGAGCCGGAGAGAGAGAGTAATTACCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATT
   
Sequence Name 12-K107427-0022-783-B09C-8409
GenBank Accession LN491606 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K107427-0022-783-B09C-8409
CGGAATGAGCCATTTACAATTGGTAATACTCTCTCTCTCCGGCTCACACGAGTCGCTAAT
CAAATAAACCTAAACCCTAGATCGTGATCGGCAACGGCACCTCCTCGTCGGCGATAGCTT
CTCTTCCACTAAAAGAAAATCCTCTTCGGTATTCTTCTTCCTCTAATCAATCTCTCTCTC
CGTTTCGGATTCTCATCTCCACTCGATGACTAGAAACGTCTCGATCTGTTTTAGATTTGA
CAACGATTCATGATTCTGATTCGTTCATTGATTTGATTCTCTTTGATTTTGTGTTAACTC
TCGTTTATAAGCTTAGATTTGGTTCTCGTCTCTGATTCTGAATCGTTCTTGTAGTGATTT
GATTTATCATATAAGCGTAGAAGAACAATTTGTGTTTAGTTGAATTTGATTTTTGTTATG
TTTAGGTGAGAATGGCAGAGCATTTAGCTTCAATCTTTGGTACTGAAAAGGATAGAGTGA
ATTGTCCGTTTTACTTTAAGATTGGTGCTTGTCGTCACGGTGACCGGTGCTCACGGCTTC
ATAACCGTCCCACCATCTCGCCGACGCTTCTCCTCTCGAACATGTACCAGAGACCTGACA
TGATAACTCCAGGTGTGGATCCTCAGGGACAGCCGTTAGACCCGAGTAAGATCCAAGACC
ACTTTGAGGATTTCTACGA

Last Updated on 06.12.2018 16:11