Sequences from confirmation amplicon


Confirmation sequences for line 393A08 and hit At5g43830 are shown.
Line ID 393A08
Locus At5g43830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17624542
Primers used for PCR 09vk - 8474, show primer details
   
Sequence Name 43-K117932-0022-393-A08C-09vk
GenBank Accession LN504990 [GenBank]
Sequencing primer gene specific primer, 09vk: 5'-ACTCAGACGAGTGTGGACTCTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K117932-0022-393-A08C-09vk
ACACGCGAGCATTTTTCTCAGTGATGATTTTCTCGGGAAAATAAAATTTGCAGAAAGAAG
AAAAAAATGGAGCAAACGAGAAAATGATGAGGAAAAAAGTGAAGAAAGACCGGGAAGGCT
ATAAAGGAATCTTGTAGGATATACGTGATTACTATTTTACCCCTACGTTATTGACACGTG
TCGTGATTCATGAGGATAAGAAAGTTTTGGATATTTTTCTTTTTTCTCAATGAGGTTAAT
AAAAAAGAGTTAATTAGATGGATAATCATATCTTTAAACGGAATCACAATTATCTGATTT
TAAACTTTTGATAAGGCAATGTAGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCACGGTTTA
   
Sequence Name 03-K117931-0022-393-A08C-8409
GenBank Accession LN487670 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117931-0022-393-A08C-8409
CTTATCAAAAGTTTAAAATCAGATAATTGTGATTCCGTTTAAAGATATGATTATCCATCT
AATTAACTGCTTTTTTATTAACCTCATTGAGAATAAAGAAAAATATCCAAAACTTTCTTA
TCCTCTTGAATCACGACACGTGTCAATAACGTACGGGTAAAATAGTAATCACGTATATCC
TACAAGATTCCTTTATGGCCTTCCCGGTCTTTCTTCACTTTTTTCCTCATCATTTTCTCG
TTTGCTCCATTTTTTTCTTCTTTCTGCAAATTTTATTTTCCCGAGAAAATCATCACTGAG
AAAAATGCGCGCTGTGTATGAGAAGACGGTGGCGAATAGCCCTGAAGTCTTTACAGAGTC
CTCACTGTATCATGAGTACGAAAAAGAAAGAGTAATTACCGTTTTTTTTTCACTTCAAAA
ATGTACATATCCGCAG

Last Updated on 06.12.2018 16:11