Sequences from confirmation amplicon


Confirmation sequences for line 031E03 and hit At5g44565 are shown.
Line ID 031E03
Locus At5g44565 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17952393
Primers used for PCR 05ma - 8474, show primer details
   
Sequence Name 41-K114180-0022-031-E03C-05ma
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 05ma: 5'-GTAATGTTGATGAATGTGACCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K114180-0022-031-E03C-05ma
CCTCTTGAACGGGAGCAGATTGTCGCGTTTATTTTGTATTGCCTGTCGGAGACTTTTATG
GAATCGTACGCTTATTATTGGCTTCATGTCCGGGAAATCTACATGGAGCTGCTCTCATTC
TGATGCGTTACATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGNTTATTA
   
Sequence Name 05-K114177-0022-031-E03C-8409
GenBank Accession LN488442 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K114177-0022-031-E03C-8409
TCATTGAAATTTCCTAAAATATAATTACAACCAATCAATATTATCGTGACTTTTTGTTTT
TGTTCTTTTGACAGCTATAATGTGTGATCTATAAAACCATCCGGTCTATAAAACCATCCG
GTCTATAAAATTTGACTGTATATCAATAATGTTATATCTGTCTACCTAAATAAAGAATAA
ATATCTTTGTTAGTTTCACAAAAAGAAAGAATAAATATCTTTAAGACTAAAATGAAGGGA
GGAGAATGATGGCGAATTATAAGCCCAATACAGCAGTAGAAACACCACCATCAAGAAGTA
GACGAGGTGGTGGTGGTCAGAATACGGTACTCACAATTCATTACTTTAAAATAAATCAAT
AATTGATCACCTCCTTAATTTAATAATTATGCACTTTGCTTCTAATATATATTAAATTTG
TCTAGTTTACGTATTCGTTATCAAATATTAATGAATTTATATCATCTTGTAGGGAGCAGA
TTGATGAGTATAATATATAAGCCAAACGGAGACATATATAATATCGGAAGCAGAGTATAT
ATATCACCTTGAAGCAGTGGACATGGTGGTGGTCACATTCTCAAACATTA

Last Updated on 06.12.2018 16:11