Sequences from confirmation amplicon


Confirmation sequences for line 700A11 and hit At5g45510 are shown.
Line ID 700A11
Locus At5g45510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18448114
Primers used for PCR FV37 - 8474, show primer details
   
Sequence Name 45-K033883-022-700-A11C-8409
GenBank Accession LN505440 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K033883-022-700-A11C-8409
AAGCCATTTACAATTGAATATATCCTGCATCAGAGGAGCTGGCATTCTCAGACCTATCAC
GTTCAATGTCTTCCTTTATTTCTCTCCATGTGTCACGTTCAAAACATTTTCCATCTGCCC
GAGTAAAACGCTTCAGACAATGGATTTTGGTATCCGCTGGCAACTCTGGTGTCTCCACTT
GGGTTCCAGACAGATCCACTACACGTAGGTAAAACATTTTCTCAAATGATCCCTCAATCT
TTGCCAGCTTGGCACTGCCGGAAACATCAATGACTTCGAGATTTTCTAGTTTCTCGATGT
TTGGGATGGCATCTAGGTTGATGCAATCACGTAGGAGGAGTTCGTTGAGACTGGACAGGT
CCTCGATGGTGGTAGCCAACTCGCTAAGATTAGTTCCCGAGAGATTAAGAGTTTTGAGTT
CAAGCTTATCCTCGAAGCACACTTCAAGCATTTCCACTAAGCTCGTAGTGCCAGAAAGAT
CAAGAATTTGAAGTCCTGACAAGGGCTTCAAACTAGGCACCTTTCTCAATAAAAGG
   
Sequence Name 05-K033883-022-700-A11C-FV37
GenBank Accession LN488252 [GenBank]
Sequencing primer gene specific primer, FV37: 5'-ATTGAGAAGGTTGCCTAGTTTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K033883-022-700-A11C-FV37
AATTCTTGATCTTTCTGAGCCTACGAGCTTAGTGGAAATGCTTGAAGTGTGCTTCGAGGA
TAAGCTTGAACTCAAAACTCTTAATCTCTCGGGAACTAATCTTAGCGAGTTGGCTACCAC
CATCGAGGACCTGTCCAGTCTCAACGAACTCCTCCTACGTGATTGCATCAACCTAGATGC
CATCCCAAACATCGAGAAACTAGAAAATCTCGAAGTCATTGATGTTTCCGGCAGTGCCAA
GCTGGCAAAGATTGAGGGATCATTTGAGAAAATGTTTTACCTACGTGTAGTGGATCTGTC
TGGAACCCAAGTGGAGACACCAGAGTTGCCAGCGGATACCAAAATCCATTGTCTGAAGCG
TTTTACTCGGGCAGATGGAAAATGTTTTGAACGTGACACATGGAGAGAAATAAAGGAAGA
CATTGAACGTGATAGGTCTGAGAATGCCAGCTCCTCTGATGCAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11