Sequences from confirmation amplicon


Confirmation sequences for line 295E12 and hit At5g45550 are shown.
Line ID 295E12
Locus At5g45550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18464210
Primers used for PCR CN59 - 8474, show primer details
   
Sequence Name 49-K104163-0022-295-E12C-CN59
GenBank Accession LN506825 [GenBank]
Sequencing primer gene specific primer, CN59: 5'-CTGCAGAAACCAAATACCAAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K104163-0022-295-E12C-CN59
GCATTGTAAAACAAACCATCTACTAAATATAAAGTGTGTTATTAAGCTGAATATTAAGCA
AAATAATACTATTTTTTTAGTTTCTTAATATTAGATGCAGAATTGTGAGATTCCACAAAA
TCACTCTATCATTTCATTATTTCGTAGATTATGAATGAGTATACTAGTAAATTTAGAACG
AGAACTCACATGAGTAAAGAGGATGAAGTGTTTGAAGCAAGTGTTGAGATGAGCTTCTTC
TTTTAGGCTAACGATTTTCTGGAAATGAGAGTGGTATATGTGAGCATAAACTCGGAATAG
CCGTTTAAAGATCGTTTTCACCACATCTTTGAAGTTCTGAGGAAATGGTGCTCCTGCAAG
AAACCAAAAATTAGCATGAGGATGTCGATAGAACATTTTGTTTGTTGATATTGATTGATC
TTCTTGTTAGATCTCATAGACATTATTATATTTACCAAGCCTTTGAGGAAATAAAGTCTC
ATCATCGAGTTGGGTCTCGATCCAATCCATCAATATGGTAATTAATCCGAAAAGGGGGTA
CCCTTTAATGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCG
   
Sequence Name 49-K104164-0022-295-E12C-8409
GenBank Accession LN506826 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K104164-0022-295-E12C-8409
TTCGGACTGAGCCTTTACATTGAAATTAAAGGGTACCCCCTTTTCGGATTAATTACCATA
TTGATGGATTGGATCGAGACCCAACTCGATGATGAGACTTTATTTCCTCAAAGGCTTGGT
AAATATAATAATGTCTATGAGATCTAACAAGAAGATCAATCAATATCAACAAACAAAATG
TTCTATCGACATCCTCATGCTAATTTTTGGTTTCTTGCAGGAGCACCATTTCCTCAGAAC
TTCAAAGATGTGGTGAAAACGATCTTTAAACGGCTATTCCGAGTTTATGCTCACATATAC
CACTCTCATTTCCAGAAAATCGTTAGCCTAAAAGAAGAAGCTCATCTCAACACTTGCTTC
AAACACTTCATCCTCTTTACTCATGTGAGTTCTCGTTCTAAATTTACTAGTATACTCATT
CATAATCTACGAAATAATGAAATGATAGAGTGATTTTGTGGAATCTCACAATTCTGCATC
TAATATTAAGAAACTAAAAAAATAGTATTATTTTGCTTAATATTCAGCTTAATAACACAC
TTTATATTTAGTAGATTGGTTTTGTTTTTTACAATTTGGCCATTTCACTGAGATTTTTGG
TATTT

Last Updated on 06.12.2018 16:11