Sequences from confirmation amplicon


Confirmation sequences for line 790A12 and hit At5g47010 are shown.
Line ID 790A12
Locus At5g47010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19074537
Primers used for PCR NC60 - 8474, show primer details
   
Sequence Name 57-K101327-0022-790-A12C-8409
GenBank Accession LN509363 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K101327-0022-790-A12C-8409
CTGCTCAAGGATACAATAGAGCCTGTTATAAATCCGTTATATAGATAACATATATTATTC
ATCGTGTTAACAAGGAACACGGGATCAAAACGACTCTGAAAATTCCGTGAAGACATTTAC
GACGAACATTATTCATGAACTGGATAAGCTGAAAGCCAATGATTACCTGAGATGCATTCA
GTTCTGGAAGACCAGGTACCCCAAAACGTCGAGGTAGTGTGTTACGAACCATCTGGGCCT
CAACTTCATGTCCCAATAACTGATGGTAGATATAACTTCAAAGAATAGAAACATAGTGAT
TCAGTGGTTGATTAGCATGCAGTAACAAATGGACACGTACCAAGAAGGAGCTAGGCTTTC
CGATGACTGACTTACCCACTCACACTTGTTTCATCTACAGCAAAATTCTTCATTGCTCCC
TGCATTCGATCAAAGCTTGTACTTTTCCAAACAAAATCAACACTGAATCCATGATTTACG
TCAATCGGAACTCCCTACACACAGAGTAAGTGAGGTTGAACATATCTCCGCAAGTCGATG
TCAAAAGCAAAGCTAACATCAGAATTATACTCACCTGATTAGCTCGGAGTTCAAGAGCAA
CCTCCTCTTGCGCCGTTAGCTTGATCTGGAGAATTATTAAAATTTTATGGTAAAGACCTA
AACACTCTCAAATA
   
Sequence Name 57-K101333-0022-790-A12C-NC60
GenBank Accession LN509364 [GenBank]
Sequencing primer gene specific primer, NC60: 5'-GTAGGGTGGAGATCCTGTATTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K101333-0022-790-A12C-NC60
ATTTTATAATTCTCCGATCAAGCTAACGGCGCAAGAGGAGGTTGCTCCTGTACTCCGAGC
TAATCAGGTGAGTATAATTCTGATGTTAGCTTTGCTTTTGACATCGACTTGCGGAGATAT
GTTCAACCTCACTTACTCTGTGTGTAGGGAGTTCCGATTGACGTAAATCATGGATTCAGT
GTTGATTTTGTTTGGAAAAGTACAAGCTTTGATCGAATGCAGGGAGCAATGAAGAATTTT
GCTGTAGATGAAACAAGTGTGAGTGGGTAAGTCAGTCATCGGAAAGCCTAGCTCCTTCTT
GGTACGTGTCCATTTGTTACTGCATGCTAATCAACCACTGAATCACTATGTTTCTATTCT
TTGAAGTTATATCTACCATCAGTTATTGGGACATGAAGTTGAGGCCCAGATGGTTCGTAA
CACACTACCTCGACGTTNTGGGGTACCTGGTCTTCCAGAACTGAATGCATCTCAGGTAAT
CATTGGCTTTCAGCTTATCCAGTTCATGAATAATGTTCGTCGTANATGTCTTCACGGAAT
TTTCAGAGTCGTTTTGATCCCGTGTTCCTTGTTAACACGATGAATAATATATGTTATCTA
TA
Primers used for PCR NC61 - 8474, show primer details
   
Sequence Name 58-K101327-0022-790-A12C-OriL
GenBank Accession LN509652 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K101327-0022-790-A12C-OriL
CTTGATCCAGGTCCACCTGGCACAGGCAAAACTGTGACTTCTGCAGCAATTGTGTATCAC
ATGGCAAAACAGGGCCAGGGACAGGTAAAGCTGATTGTTATTGATTCCCTCAAGATTGCT
TTTTGTGAGTTGACTATCAGATATTTATTATTATTACCTGACAGGTTCTCGTTTGTGCCC
CAAGTAATGTTGCTGTGGACCAACTAGCTGAAAAAATTAGTGCTACTGGGTTAAAGGTAA
ACTTTTAAACCTCCTAGTTTTTCTATTAAAATTTTGCGTAAGACATGGATACTTCAATTT
ATTGTTCATTTTTGGAAGTTTCTACCATCAAAATTTATTTAAAGAGTATATGACTTCTCT
TCAAACTTTCTCTTACTCTAATGTCAGGTTGTTCGCCTTTGCGCAAAATCAAGGGAAGCT
GTAAGTTCTCCTGTAGAGTATTTGACCCTTCACTACCAGGTTAGTTGACATGGTAGCACG
CTNTGGTTCATTTAACTCTCTGG
   
Sequence Name 58-K101333-0022-790-A12C-NC61
GenBank Accession LN509653 [GenBank]
Sequencing primer gene specific primer, NC61: 5'-CCAGAGAGTTAAATGAACCAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K101333-0022-790-A12C-NC61
GAGGGTCAAATACTCTACAGGAGAACTTACAGCTTCCCTTGATTTCGCTCAAAGGCGAAC
AACCTGACATTAGAGTAAGAGAAAGTTTGAAGAGAAGTCATATACTCTTTAAATAAATTT
TGATGGTAGAAACTTCCAAAAATGAACAATAAATTGAAGTATCCATGTCTTACGCAAAAT
TTTAATAGAAAAACTAGGAGGTTTAAAAGTTTACCTTTAACCCAGTAGCACTAATTTTTT
CAGCTAGTTGGTCCACAGCAACATTACTTGGGGCACAAACGAGAACCTGTCAGGTAATAA
TAATAAATATCTGATAGTCAACTCACAAAAAGCAATCTTGAGGGAATCAATAACAATCAG
CTTTACCTGTCCCTGGCCCTGTTTTGCCATGTGATACACAATTGCTGCAGAAGTCACAGT
TTTGCCTGTGCCAGGTGGACCTTGGATCAAGCTGATGGGCTTCTGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11