Sequences from confirmation amplicon


Confirmation sequences for line 732G05 and hit At5g49680 are shown.
Line ID 732G05
Locus At5g49680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20181266
Primers used for PCR 70ra - 8474, show primer details
   
Sequence Name 46-K115914-0022-732-G05C-70ra
GenBank Accession LN505966 [GenBank]
Sequencing primer gene specific primer, 70ra: 5'-GAAAGCTGTTGAGAGAAGAGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K115914-0022-732-G05C-70ra
ATAAAGCCGCGAAAAAGGTATGTCTTTTTTCTTCAGCTACAGGATCAAGCTTCTTAACAA
TTTCTATCATCCCAGCTTCACCTCCACCAATAGCTGTCAAACTTAGATCCAAATCAGTAA
CAGAGACAGAAAGTAGAGATGTCCTAGAAGTGCTCATCTTAAAACCAGCCTGAAAACCTT
CTTTACAGGCACCTGAACCATCTGAAAGTCTTAAACTTTGACAAGACTTGTAATATGAAC
TGAACGACTGTTTATATAATTTTTCAATTATAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 20-K115913-0022-732-G05C-8409
GenBank Accession LN495357 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K115913-0022-732-G05C-8409
AAGAAGGTTTTCAGGCTGGTTTTAAGATGAGCACTTCTAGGACATCTCTACTTTCTGTCT
CTGTTACTGATTTGGATCTAAGTTTGACAGCTATTGGTGGAGGTGAAGCTGGGATGATAG
AAATTGTTAAGAAGCTTGATCCTGTAGCTGAAGAAAAAGACATACCTTTTTCGCGGCTTT
ATGGAAGCAATCTTCGGTTAAACACTGGAACTCTCGCTGTTCAAATACGAAATTACACAT
TTCCTCTTCTTAAACACAGCTT

Last Updated on 06.12.2018 16:11