Sequences from confirmation amplicon


Confirmation sequences for line 734G06 and hit At5g51810 are shown.
Line ID 734G06
Locus At5g51810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21055671
Primers used for PCR OR04 - 8474, show primer details
   
Sequence Name 57-K027349-022-734-G06C-8409
GenBank Accession LN509300 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K027349-022-734-G06C-8409
ATTGAATATATCCTGTATGAGTTGATTAACCATTTCTGTATGAGTAACCAAAATGTGTTT
ACTTTCTTAGAATGTTTTCAATTACCATGAAAGTGTCACCAATATTGACAACGAAAGCCT
TGGGATTGGGACGAATGGATTGCCATTGATTGTCGACAAAGACTTGAAGGCCATTGACAT
GGTCTTGATGAAGGATGGTCAAAGAACTTGGATCACAATGAGGTCCTGTACCTAACGTGA
GATCTGGTGTTTGGCATGGAGGATAATGATTGAGCCTCATTATCGAATCGTTCTCTTCGA
AAAATCCTCGGAAATAGTCTCGGTTTACGCCTAAACTTAAGCCCAGAAGCTCCATGATCT
TGAGTGATAGAGAACTCATTGCTTCACAATAGTCTTGATACACCTTCCTGTAAAGAAGAC
CGGGAAAATCATTAGGTAAACCGGTCAAATTATTTCCGGTATAACAAGCCGGTTTATTTA
GGTCAGACTAGAAAACAGAGAAAATAAGGTTTTGCTCTGTTTTGTGGTTCTGTTTTAAAA
AGACACGTACCCAAACTGCTCGAACTCTTGTCCTAATGTATCGGAAAAGTAATCTT
   
Sequence Name 58-K027349-022-734-G06C-OR04
GenBank Accession LN509591 [GenBank]
Sequencing primer gene specific primer, OR04: 5'-TCGAGAACCGTTCAAGATTACTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K027349-022-734-G06C-or4
GGTCGTGTCTTTTTAAACAGAACCACAAAACAGAGCAAAACCTTATTTTCTCTGTTTTCT
AGTCTGACCTAAATAAACCNGGCTTGTTATACCGGAAATAATTTGACCGGTTTACCTAAT
GATTTTCCCGGTCTTCTTTACAGGAAGGTGTATCAAGACTATTGTGAAGCAATGAGTTCT
CTATCACTCAAGATCATGGAGCTTCTGGGCTTAAGTTTAGGCGTAAACCGAGACTATTTC
CGAGGATTTTTCGAAGAGAACGATTCGATAATGAGGCTCAATCATTATCCTCCATGCCAA
ACACCAGATCTCACGTTAGGTACAGGACCTCATTGTGATCCAAGTTCTTTGACCATCCTT
CATCAAGACCATGTCAATGGCCTTCAAGTCTTTGTCGACAATCAATGGCAATCCATTCGT
CCCAATCCCAAGGCTTTCGTTGTCAATATTGGTGACACTTTCATGGTAATTGAAAACATT
CTAAGAAAGTAAACACATTTTGGTTACTCATACAGAAATGGTTAATCAACTCATACAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCG
ACGTGTCTA

Last Updated on 06.12.2018 16:11