Sequences from confirmation amplicon


Confirmation sequences for line 217E06 and hit At5g53135 are shown.
Line ID 217E06
Locus At5g53135 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21543359
Primers used for PCR 18xm - 8474, show primer details
   
Sequence Name 89-K119874-0022-217-E06C-18xm
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 18xm: 5'-AGATCGCCTTGTCCTGTAATAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K119874-0022-217-E06C-18xm
TGATGCAGATGATGATGCAGATGCATGATTAGGCAGTGTATGATGCACAGGTAAAGTATC
AAGAGCAACAGGAGTAGACATAGGTAATATAATGTGATGAAATAAATCCTCAGTAGGCAA
GTTAGAAGGAACATTTCTAAATGGAAAAGATGTTTCATGAAAAATAACATTCCTTGAAAT
GGATATAACATTTGTACTCAAATTCATCACCTTATAACCTTTGTAACCAGATGGATAACC
TAGAAAAACACATGGTTCAGCCCTAGGTGTAAACTTAGTCCTATCTTTGACTAGAGTAGA
CACATAACACAAGCAACCAAAAGATCTCAAGAAATTATAATCTGGTTTCTTTTTGAAAAG
CTTTTCATATGGAGATATTTTCTGTAAAATCAAAGAAGGAGTTCTATTAATCAGAAATAC
TGCAGTTTGAATGCATTCACTCCAATAGACCAAAGGCATATTAGATTGAAACAAAAGAGC
ACGAGCTACATTGAGTAAATGCTGATGTTTTCGCTCGACCACAGAGTTCTGTTGAGGAGT
ATATACACATGAGAAATGATGTATCATACCAGTATCTTTAACGAGTTGAGTGAAAGCCAA
TTCAGGTGCATTATCACTACGAATAGCCTTAATGATCGAATTATACTGTGTATGAACATA
TTTAACAAAGACAGGAAAAATTTTAGTAACTTCACTTTTATTCCTTAGCATATAGACCCA
AGTAACTCTAGTACAATCATCAACAATAGTGAGAAAATACTTAAAACCATCTATAGACTC
TACTGAGAATGGACCCCAAACGTCTAAATGAATAAGATCAAATGGATTCTCANGACAATG
ATTATTTGAAACTAAAAGGTACCCTTTTCTTGTTTAGCTATGGACAGACTTCACAATGAT
CATGAATAGCAGACTTAGTCATAGGTAAAAT
   
Sequence Name 65-K119873-0022-217-E06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K119873-0022-217-E06C-8409
ATATGTGCAGTTTCATATTTACGCTACGCACAGTGCATATTTCATCCACATTGACTTTGC
ATAATGTTTTATATGTTTCAACATTCAAGTTTAATCTCATCAGTGTTAGTTCTTTACTTA
TGCATAATAAATGTTCAGCTCATTTCTATCATGATTCATGTTATATTCAGGACCATACTC
GGGACTTGACGATTGGTAGAGGAACTCTCATACATAATCTTTACATTCTGAAGAAAGAAG
ATACATTGCATTCATCAAATTTCTGTGGATCCTTGATAGTTGATGATCGTCTATGGCATC
ATCGCCTAGGACATCCATCATCTGCCAAGTTACAACATCTTTCTGGTATTTTACCTATGA
CTAAGTCTGCTATTCATGATCATTGTGAAGTCTGTCCATTAGCTAAACAGAAAAGGTTAC
CTTTTAGTTCAAATAATCATTTGTCTGAGAATCCATTTGATCTTATTCATTTAGACGTTT
GGGGTCCATTCTCAGTAGAGTCTATAGATGGTTTTAAGTATTTTCTCACTATTGTTGATG
ATTGTACTAGAGTTACTTGGGTCTATATGCTAAGGAATAAAAGTGAAGTTACTAAAATTT
TTCCTGTCTTTGTTAAATATGTTCATACACAGTATAATTCGATCATTAAGGCTATTCGTA
GTGATAATGCACCTGAATTGGCTTTCACTCAACTCGTTAAAGATACTGGTATGATACATC
ATTTCTCATGTGTATATACTCCTCAACAGAACTCTGTGGTCGAGCGAAAACATCAGCATT
TACTCAATGTAGCTCGTGCTCTTTTGTTTCAATCTAATATGCCTTTGGTCTATTGGAGTG
AATGCATTCAAACTGCAGTATTTCTGATTAATAGAACTCCTTCTTTGATTTA

Last Updated on 06.12.2018 16:11