Sequences from confirmation amplicon


Confirmation sequences for line 208B03 and hit At5g53290 are shown.
Line ID 208B03
Locus At5g53290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21619064
Primers used for PCR WD01 - 8474, show primer details
   
Sequence Name 17-K017178-022-208-B03C-WD01
GenBank Accession LN493654 [GenBank]
Sequencing primer gene specific primer, WD01: 5'-TTATCTGACCGGAGACTGAAACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K017178-022-208-B03C-WD01
TGACGACTCGACGCCGGAGATTGAGTTTCATCGGAGGAAGAAGAGAGTCTCTTTCTATCT
TTCATCGAAACTCCGGTGACGACGTTGTTACAGCTAGGCTCAACAGTGATCTCGTTAACG
AATCTTTTGACTCTCCGGCGAGGAAACAAGAAATCTTCTTCGTCTTCGTCACTTGATGAA
TCTGTTGCGTCAGGATCAGTATAACAAATACTAACCTTTTTCAATGACTTACCACCGGAA
AGTTTTTCCGATGACTTTTTGGTGTATTTAGTCATTGAAGTCTTGTGCTCTGTGTACTTC
AATGGTCGGAAATCAATATATTCGTCCATTACTGTTTCTTTTATGGACTCTCTCTATATT
CGCATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGGTCTACAT
   
Sequence Name 21-K017178-022-208-B03C-8409
GenBank Accession LN495453 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K017178-022-208-B03C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGATGCGAATATAGAGAGAGTCCAT
AAAAGAAACAGTAATGGACGAATATATTGATTTCCGACCATTGAAGTACACAGAGCACAA
GACTTCAATGACTAAATACACCAAAAAGTCATCGGAAAAACTTTCCGGTGGTAAGTCATT
GAAAAAGGTTAGTATTTGTTATACTGATCCTGACGCAACAGATTCATCAAGTGACGAAGA
CGAAGAAGATTTCTTGTTTCCTCGCCGGAGAGTCAAAAGATTCGTTAACGAGATCACTGT
TGAGCCTAGCTGTAACAACGTCGTCACCGGAGTTTCGATGAAAGATAGAAAGAGACTCTC
TTCTTCCTCCGATGAAACTCAATCTCCGGCGTCGAGTCGTCAACGTCCTAATAACAAAGT
TTCAGTCTCC

Last Updated on 06.12.2018 16:11