Sequences from confirmation amplicon


Confirmation sequences for line 142F07 and hit At5g53450 are shown.
Line ID 142F07
Locus At5g53450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21690872
Primers used for PCR BU15 - 8474, show primer details
   
Sequence Name 02-K104372-0022-142-F07C-BU15
GenBank Accession LN487017 [GenBank]
Sequencing primer gene specific primer, BU15: 5'-TAATGAGGGATATTTTGATCGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K104372-0022-142-F07C-BU15
TTCTACTTTTCGTTGTGATCTTCTTAACATGTTTTTTGGTGTTTGTTCAAACTTGAAGGT
CAATTACTTGCACAGCCATGGTCTGGCTCACACAGAACTGAGATTGGAAAATGTGCATAT
TAGCCCTGTGGATAGACATATCAAAGTTAGTTTAGACTTTGAGTAGTGAAGTTTTATTAT
TCATGTTTCAGTTTTGTCAATTAAAATCAGAGTTCATTTCCGACTATAGGTAGGGATTCT
CGGAAATGCTGCTGACTTTAACGGGGATGTTCCAAGTACTAGCAACGCTTACAGTACCAT
GGACAGACGACAAATGATGATAGCATTTGACATGAGGTATTTTGAGAAGAGATCGTAATT
ATAATATCTGTTTGTTGACATTACTCGGCTTAATTTTAAATTTCTTTTTCATGATCAAAG
ATGTGTTGGATTCATGATGGCAAAAATGGTACTTCAAGAATTGATGGATCCATTAATCTT
TGCGAAATTGAAGTCTTTCCTGGCAAAGGTATTATTACTTAGGCTACATAGTTTGATGTT
ATGAATTGTATGTTGATTTTCGTTAAATTTTCTTCTAAATATAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCAC
   
Sequence Name 18-K104373-0022-142-F07C-8409
GenBank Accession LN494289 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K104373-0022-142-F07C-8409
TTATATTAGAAGAAATTTAACGAAAATCAACATACAATTCATAACATCAAACTATGTAGC
CTAAGTAATAATACCTTTGCCAGGAAAGACTTCAATTTCGCAAAGATTAATGGATCCATC
AATTCTTGAAGTACCATTTTTGCCATCATGAATCCAACACATCTTTGATCATGAAAAAGA
AATTTAAAATTAAGCCGAGTAATGTCAACAAACAGATATTATAATTACGATCTCTTCTCA
AAATACCTCATGTCAAATGCTATCATCATTTGTCGTCTGTCCATGGTACTGTAAGCGTTG
CTAGTACTTGGAACATCCCCGTTAAAGTCAGCAGCATTTCCGAGAATCCCTACCTATAGT
CGGAAATGAACTCTGATTTTAATTGACAAAACTGAAACATGAATAATAAAACTTCACTAC
TCAAAGTCTAAACTAACTTTGATATGTCTATCCACAGGGCTAATATGCACATTTTCCAAT
CTCAGTTCTGTGTGAGCCAGACCATGGCTGTGCAAGTAATTGACCTTCAAGTTTGAACAA
ACACCAAAAAACATGTTAAGAAGATCACAACGAANAGTAAGAAATATGCTGCTTGTTCGA
AGGACCAAATTACATACTCCGATCA
Primers used for PCR BU16 - 8474, show primer details
   
Sequence Name 03-K104372-0022-142-F07C-BU16
GenBank Accession LN487473 [GenBank]
Sequencing primer gene specific primer, BU16: 5'-TCTTCTGAAATCTTTACTGCGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K104372-0022-142-F07C-BU16
CATTGATGGGGCACACGCCATCTTGGTCCACATAGAAAGGGATGCTTAAGAGCATCCAAG
CAACTGCACCAAACCCCAACAGAATTTATAAATGTAGCTTCTGAGTGTTTAGCCTATAGA
TTGGTAATACTATCGTGCACTAATGTTATGTTACCTTATTCTTTCAGAAGGTCTGGTAGC
AATTAACAAAGATAAAAGGTGCCAACCTGCTCCCCAGTTTCTATCAAGTATCTGAGAGAA
AAAAAACATTTGCTAAATCTCACATCGAATGAAAGAACTAGTAATAGCACTTAGCTGATT
ACTTACTTGCACTCCGGTATTTCCAGATTCAGAGTTTGTATTGAGCGTCGTCACAAAAAA
CTCCCGCAGCGAGGAAGGATCATTCCCCTATAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 19-K104373-0022-142-F07C-3144
GenBank Accession LN494745 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K104373-0022-142-F07C-3144
GGGGAATGATCCTTCCTCGCTGCGGGAGTTTTTTGTGACGACGCTCAATACAAACTCTGA
ATCTGGAAATACCGGAGTGCAAGTAAGTAATCAGCTAAGTGCTATTACTAGTTCTTTCAT
TCGATGTGAGATTTAGCAAATGTTTTTTTTCTCTCAGATACTTGATAGAAACTGGGGAGC
AGGTTGGCACCTTTTATCTTTGTTAATTGCTACCAGACCTTCTGAAAGAATAAGGTAACA
TAACATTAGTGCACGATAGTATTACCAATCTATAGGCTAAACACTCAGAAGCTACATTTA
TAAATTCTGTTGGGGTTTGGTGCAGTTGCTTGGATGCTCTTAAGCATCCCTTTCTATGTG
GACCAAGATGGCGTGTTGCCCCATCAATGGATATCATCAGATGGGGTCTTGGATCAACCG
CAGTAAAGATTTCAGA

Last Updated on 06.12.2018 16:11