Sequences from confirmation amplicon


Confirmation sequences for line 907C12 and hit At5g54390 are shown.
Line ID 907C12
Locus At5g54390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22086886
Primers used for PCR 43xr - 8474, show primer details
   
Sequence Name 54-K119546-0022-907-C12C-43xr
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 43xr: 5'-CTCTTCCTTTCTCCTCCGATAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K119546-0022-907-C12C-43xr
TTGACCGGCGGTGCGTGTTGTCCACCTCGCTTCTTCTCTCTGTGTTAAAGTTCAAGAGAA
GCTTCATCTTCCTAACGGTGGTCACGTTAAGTCTAAAGACGATGATTCCCCTGTCACCGT
CGCTGGTTCGATTTCTCTGTTTCTCTGTTTTGTGTTCTGTTTTTTTCTAATTGTAATTTT
GAGATCTGTTTGTGTTAAATAAAAAAAAAACGAATTTTTGATGTTGGATTGTGTAATCGA
ATAATCATGTTCTTTAATTCGTTGATAGATGAAACAAAATGTGTATTAAAATACAAAAGT
TTGCAACTTTTCATCACTTCCAATGATTCTCTGTATTAGTAGTTCAATAGTGTATCAAAG
TTTTAAACTTTTGTTGATTCACTTGACAGATTTTGGTGTACAAGCAATTGTGAGCTGGGT
TTTAGCTGAAGTGTTTGGTGATCAAAACCTTTCAATTGTTGCTGAAGAAGACACTGAGAC
ACTCTCTGAGGCTGATTCTTTAGGTCTTTTAGGAGCTGTGTCGAATGCGGTTAATGAAGC
ATTGTCCGAAGCTCAAAACTACGGGCTTCCGAAGCCAGTTAAGCCATTGGGGTCTAGTGA
AATTCTTAAGGCTATTAGTAGATGTAACTCTGTTGGAGGACCTAAAGGAAGGCATTGGGT
TCTTGATCCTGTTGATGGAACGTTAGGGTTTGTTCGTGGGGATCAGTATGCTGTTGCTTT
AGCTTTGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGGAAAAA
   
Sequence Name 22-K119545-0022-907-C12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K119545-0022-907-C12C-8409
CATTTACATTGAATTATCCTGATCAAGCTAAAGCAACAGCATACTGATCCCCACGAACAA
ACCCTAACGTTCCATCAACAGGATCAAGAACCCAATGCCTTCCTTTAGGTCCTCCAACAG
AGTTACATCTACTAATAGCCTTAAGAATTTCACTAGACCCCAATGGCTTAACTGGCTTCG
GAAGCCCGTAGTTCTGAGCTTCGGACAATGCTTCATTAACCGCATTCGACACAGCTCCTA
AAAGACCTAAAGAATCAGCCTCAGAGAGTGTCTCAGTGTCTTCTTCAGCAACAATTGAAA
GGTTTTGATCACCAAACACTTCAGCTAAAACCCAGCTCACAATTGCTTGTACACCAAAAT
CTGTCAAGTGAATCAACAAAAGTTTAAAACTTTGATACACTATTGAACTACTAATACAGA
GAATCATTGGAAGTGATGAAAAGTTGCAAACTTTTGTATTTTAATACACATTTTGTTTCA
TCTATCAACGAATTAAAGAACATGATTATTCGATTACACAATCCAACATCAAAAATTCGT
TTTTTTTTTATTTAACACAAACAGATCTCAAAATTACAATTAGAAAAAAACAGAACACAA
AACAGAGAAACAGAGAAATCGAACCAGCGACGGTGACAGGGGAATCATCGTCTTTAGACT
TAACGTGACCACCGTTAGGAAGATGAAGCTTCTCTTGAACTTTAACAC

Last Updated on 06.12.2018 16:11