Sequences from confirmation amplicon


Confirmation sequences for line 765H11 and hit At5g54690 are shown.
Line ID 765H11
Locus At5g54690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22220735
Primers used for PCR 20kn - 8474, show primer details
   
Sequence Name 70-K113756-0022-765-H11C-20kn
GenBank Accession LN512426 [GenBank]
Sequencing primer gene specific primer, 20kn: 5'-AAAGCCAAAGCTTTTAGGAAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K113756-0022-765-H11C-20kn
ACTTCGTTTCGATTATTGAGCGCATTTATTTTTACTATGGTGAAACAGAGCGGTGAAAAG
TTAAGTTTTTGGTTATTTCTAGAATGTTCCAGAAGCTATGTACCAAGTTTTAGAACAGCC
TTTAAGCGAACAAGAACTCAAAGGAAGATCAGATATACCTCAAACACTTCAAGATTTCAT
GTCTGAAGTCAAAAGAAGCAAATCAGACGCAAGAGAATTTGCTCAAAAGCTAAAAGAAAT
GGTGACATTGATGGAACAGAGAACAAGAACGGCTAAGATTCAAGAGTATGATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
   
Sequence Name 32-K113755-0022-765-H11C-8409
GenBank Accession LN500663 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K113755-0022-765-H11C-8409
CTCTTGATCTTAGCCGTTCTTGTTCTCTGTTCCATCAATGTCACTATTTCTTTTAGCTTT
TGAGCAAATTCTCTTGCGTCTGATTTGCTTCTTTTGACTTCAGACATGAAATCTTGAAGT
GTTTGAGGTATATCTGATCTTCCTTTGAGTTCTTGTTCGCTTAAAGGCTGTTCTAAAACT
TGGTACATAGCTTCTGGAACATTCTAGAAATAACCAAAAACTTAACTTTTCACCACTCTG
TTTCACCATAGTAAAAATAAATGCGCTCAATAATCTGAAACTGAAGTTAGAGAGGACTTA
CACCAGAATCAACCCTTCTTCCTAAAAGCTTTGGCTTTA

Last Updated on 06.12.2018 16:11