Sequences from confirmation amplicon


Confirmation sequences for line 659B02 and hit At5g57320 are shown.
Line ID 659B02
Locus At5g57320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23215088
Primers used for PCR 31of - 8474, show primer details
   
Sequence Name 43-K114954-0022-659-B02C-31of
GenBank Accession LN504941 [GenBank]
Sequencing primer gene specific primer, 31of: 5'-ATCTCCAGGTAGTGACGATGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K114954-0022-659-B02C-31of
CAAACATAGTGTTTTGATTGCTAACTTTAAAAAATTCAGGTCTGGCGAATCAATTGTGAA
GAAAAGATCCTTCTCGAAGCAGCAGAGCAATCAAAGTTCTATAGCGGGGATTGTTATATA
CTCCAGTACTCATACCCAGGAGAAGACAGAGAGGAACATCTAGTGGGTACTTGGTTTGGC
AAGCAAAGCGTTGAGGTAAATTTCTTCACACACACACACACACATATATATATATATATA
CCCTTCTCACTCTCTATATGTTCTCATTGATTATGAGTTATATAGGAAGACAGAGCATCT
GCTATTTCTTTGGCAAACAAGATGGTTGAATCCATGAAGTTTGTGCCGGCTCAGGTGCTT
CTTCCTGTTGTTTCCGTTATAGATGCTAGGGTCGATTTACGTTATGGAAAAGCAATGAGT
ATGATATATTCAATTATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCA
   
Sequence Name 09-K114953-0022-659-B02C-8409
GenBank Accession LN490304 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114953-0022-659-B02C-8409
TTATATTGAATATATCATACTCATTGCTTTTCCATAACGTAAATCGACCTTAGCATCTAT
AACGGAAACAACAGGAAGAAGCACCTGAGCCGGCACAAACTTCATGGATTCAACCATCTT
GTTTGCCAAAGAAATAGCAGATGCTCTGTCTTCCTATATAACTCATAATCAATGAGAACA
TATAGAGAGTAAGAAGGGTATATATATATATATATGTGTGTGTGTGTGTGTGAANAAAT

Last Updated on 06.12.2018 16:11