Sequences from confirmation amplicon


Confirmation sequences for line 572D02 and hit At5g57350 are shown.
Line ID 572D02
Locus At5g57350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23234748
Primers used for PCR 64qf - 8474, show primer details
   
Sequence Name 45-K115550-0022-572-D02C-64qf
GenBank Accession LN505638 [GenBank]
Sequencing primer gene specific primer, 64qf: 5'-TCTCTCTCTCTTTTTCCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K115550-0022-572-D02C-64qf
CAGCTAATGACCTAAGTCCACGCTCAGCGTACTTATCGATTGTAGAGTGGACTCTCTTCC
TCAGATCAGCTCTCGCGTTACAGAGATCAAGAATCTGAAATCATCAAAGAATCAGTTTTT
TTAAGAAGTCACAACACTTAACTTAGCTTAAGAACCAAAATCAGTTTTAGTTACCTGCTC
GGGAGCTCCTTTGCTTACTCGGTGCCAGTTTCCGTTGCTGTCGATAAACGTCAAAGCGGT
TCGCTTATCAACTGGATTGAATGGTAGGAAGTGAATCTCTCTGATTCCAGCTCTTGCCTG
AAAGAAACAAACAATGAGAAACAGAACAGAACATTTTCTTCAAGAAACAAAACAGAGGAA
AAAACAGAATTTTAAAACAGAGGAAAAAACAGAGTTATATATATTACCTCTTTTGGATCA
GCGAGCATTCCAACCATAGCAGCATCAATAGCATCTTGGTTCTCAACTCTTGAAGCTCTA
GCAGCAAAAAGCAAAACTTCATCTTTCTCAACGCCTTTACAATAAACCTCAATAAGATTC
TTGTCAACACTAAGCTTGTTTAGCGTGAGAGTCCCGGTTTTATCGCTGCACAGAACATCC
ATACCAGCCATTTCTTCAATGGCGGTCATTCGTTTTGTGATAGCTCCTTGTTGAGACAAC
TTGTGAGATCCAATAGCCATTGTGACAGACAAGACAGTAGGCATTGCAATGGGGATACCA
CCAATCAAAAGAACAAGAAGATTGTCGATTCCGTCTCTGTAGTGTCTGCGCTGGATCGGA
TACATGACAACGATCTCAATCGCAATACCAACAGCTATGGAACATATGCAGAAGTTTCCA
ATCGCCGTAAGAACTTTCTGGAAATGTCCAACTTGGTTTGTGCTGTCGACTAGATGAGCC
GCTNTACCGAAGAAAGTATGGACTTCCGTGGCTATCACAACCGCTTCAATCTCACCTTGC
TTGC
   
Sequence Name 18-K115549-0022-572-D02C-8409
GenBank Accession LN494439 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115549-0022-572-D02C-8409
ACTTGAAATGTTTGGCATAAGTAATTGGTTNACATATAATTTCATTACAAGCTTTTAATA
ATATTTGTTTTGTTATGACCAGGTTCTAAGAGATGGCAAATGGTCAGAGCAAGAAGCTTC
TATTCTTGTTCCTGGAGATATTGTGAGCATCAAGCTTGGTGACATCATTCCTGCTGATGC
ACGTCTTCTTGAAGGTGATCCTTTAAAAGTCGACCAATCTGCTTTGACCGGAGAATCTCT
TCCCGCAACGAAAGGTCCAGGGGAAGAAGTTTTCTCCGGTTCTACCTGCAAGCAAGGTGA
GATTGAAGCGGTTGTGATAGCCACTGGAGTCCATACTTTCTTCGGTAAAGCGGCTCATCT
AGTCGACAGCACAAACCAAGTTGGACATTTCCAGAAAGTTCTTACGGCGATTGGAAACTT
CTGCATATGTTCCATAGCTGTTGGTATTGCGATTGAGATCGTTGTCATGTATCCGATCCA
GCGCAGACACTACAGAGACGGAATCGACAATCTTCTTGTTCTTTTGATTGGTGGTATCCC
CATTGCAATGCCTACTGTCTTGTCTGTCACAATGGCTATTGGATCTCACAAGTTGTCTCA
ACAAGGAGCTATCACAAAACGAATGACCGCCATTGAAGAAATGGCTGGTATGGATGTTCT
GTGCAGCGATAAAACCGGGACTCTCACGCTAAACAAGCTTAGTGTTGACAAGAATCTTAT
TGAGGTTTATTGTAAAGGCGTTGAGAAAGATGAAGTTTTGCTTTTTGCTGCTAGAGCTTC
AAGAGTTGAGAACCAAGATGCTATTGATGCTGCTATGGTTGGAATGCTCGCTGATCCAAA
AGAGGTAATATATATAACTCTGTTTTTTCCTCTGTTTTAAAATTCTGTTTTTTCCTCTGT
TTTGTTTCTTGAAGAAAATGTTCTGTTCTGTTTCTCAATGTTTGTTTCTTTCA

Last Updated on 06.12.2018 16:11