Sequences from confirmation amplicon


Confirmation sequences for line 310D11 and hit At5g60350 are shown.
Line ID 310D11
Locus At5g60350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24278546
Primers used for PCR 29uw - 8474, show primer details
   
Sequence Name 37-K117497-0022-310-D11C-29uw
GenBank Accession LN502764 [GenBank]
Sequencing primer gene specific primer, 29uw: 5'-AAAGATGGGAGAATGGTAGGAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K117497-0022-310-D11C-29uw
GATAGTTAGAGGATAGTAATTTGAGTTAATTAGGAGTTTGTTCATTTGACTGAAAAACGT
CGGGGCCAAGCAATTGATTTCAATCCCTTACCGCTACTCTTTATTACTCCTTTCAGATCA
AACGTCTTCACCTTGTTAAAGTCATTCCTCATAGGATGACTCGGTGGAGCACAGATTCCC
ATATTAATTGGTGCTGCGGACACGTGATATCCCTGACCCCGAGCTGATGGGGAGGGTACA
TAAGTTGGCTGCCTTGGCCGAGTAACTGCTACAACACAAAATGGAACATATAAGAATCAA
TCTATTTGCTACAAAGTACAAACAAACTAGTGCATGAGATACTTCCAAGTGCAGAATTAG
CACTTGAGAATATGTCAATATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 05-K117496-0022-310-D11C-8409
GenBank Accession LN488530 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117496-0022-310-D11C-8409
TATTCTGCACTTGGAAGTATCTCATGCACTAGTTTGTTTGTACTTTGTAGCAAATAGATT
GATTCTTATATGTTCCATTTTGTGTTGTAGCAGTTACTCGGCCAAGGCAGCCAACTTATG
TACCCTCCCCATCAGCTCGGGGTCAGGGATATCACGTGTCCGCAGCACCAATTAATATGG
GAATCTGTGCTCCACCGAGTCATCCTATGAGGAATGACTTTAACAAGGTGAAGACGTTTG
ATCTGAAAGGAGTAATAAAGAGTAGCGGTAAGGGATTGAAATCAATTGCTTGGCCCCGAC
GTTTTTCAGTCAAATGAACAAACTCCTAATTTAACTCAAATTACTATCCTCTTAACTATC
TGTATAATTTTATCTCTTCAAAATCACTCAAACAACTTCCTACCATTCCCCCATCTTT

Last Updated on 06.12.2018 16:11