Sequences from confirmation amplicon


Confirmation sequences for line 867A06 and hit At5g60620 are shown.
Line ID 867A06
Locus At5g60620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24367946
Primers used for PCR JD20 - 8474, show primer details
   
Sequence Name 74-K102874-0022-867-A06C-8409
GenBank Accession LN513051 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K102874-0022-867-A06C-8409
ATTTACGGGATAAACAATGAAAGGAAAATAATCCACCCAAAAGCTAAAGTGAAGCACCTG
CAAGTTTTAAAACAATGAAACTGCTCAGTACAAACATGCAGATCTAAACTTTGAGCTCTG
GAGAGGATAAGAGCCATATATAATTGAATAAATAAGAGGAACCAACCTCAAGGGAAAGAG
GATACAGTATCTAACAACAACCCCAAAGCAGTATAGTGGGAATAAGTAAATATTCCAGTT
CCAAGGTTCTGGAGGATTTGATTTGAAACATCTTGTGAACGAGTCCTGCAACAATCAAAC
CCACAAAACAAAACATCTTATACTCAAAAATCTATCCTTTATGTGATAAATTTGAAGTCT
TTTGATAGAGTAATGGTGAGACAGATTTGGGT
   
Sequence Name 74-K102875-0022-867-A06C-JD20
GenBank Accession LN513052 [GenBank]
Sequencing primer gene specific primer, JD20: 5'-AACCCAAATCTGTCTCACCATTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K102875-0022-867-A06C-JD20
TTAATTTTCCATAAAGGATAGATTTTTGAGTATAAGATGTTTTGTTTTGTGGGTTTGCTT
GTAAGGGGACTCGTTCACAAGATGTTTCAAATCAAATCCTCCAGAACCTTGGAACTGGAA
TATTTACTTATTCCCACTATACTGCTTTGGGGTTGTTGTTAGATACTGTATCCTCTTTCC
CTTGAGGTTGGTTCCTCTTATTTATTCAATTATATATGGCTCTTATCCTCTCCAGAGCTC
AAAGTTTAGATCTGCATGTTTGTACTGAGCAGTTTCATTGTTTTAAAACTTGCAGGTGCT
TCACTTTAGCTTTTGGGTGGATTATTTTCCTTTCATTGTTTATCCCTGTAAATGGCTTCC
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATTGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGAGTCTACATTCACGTCCAAAT
GGG

Last Updated on 06.12.2018 16:11