Sequences from confirmation amplicon


Confirmation sequences for line 594F06 and hit At5g60920 are shown.
Line ID 594F06
Locus At5g60920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24513473
Primers used for PCR 06ci - 8474, show primer details
   
Sequence Name 83-K107938-0022-594-F06C-06ci
GenBank Accession LN514322 [GenBank]
Sequencing primer gene specific primer, 06ci: 5'-GTTACCTTGTTCAGTTGTTTGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K107938-0022-594-F06C-06ci
TTCTTCTTGCCCATTTCACCTAATGTCCATCCTGGAGATTGAATGTGTCTGTATTTCTGG
AAGTTGAACATCGTAACCACGGCCTAGATCGAACAAACAAACAGTTTAGATGAATCAAAA
ACCGGTAAACAAGGCCTAATATTTTCTACTTCAGGTTCTTGAACTTACAACATAGCCATC
AGGAGTCCAGCTCATAACATCCCATTTCATTGTAATGTTGCCTTCTGGATCAAGCGCATC
ATATGCTTCTGCAAAAGGATTTACCAAAAGTCAGAACTTTGTTTATCCTGATCCACACCG
ACATTGTGATTGCTTCAATCTTAAAACTACTAATTATTTGGTTTGTAGAAAATAGACCAA
AGTGTTAACTGCTTATAACTCACAAGTGTGCATCAAGTTTTCTACTTATAGAAAATATCA
GAAAGTTACTAATTTTAGATCAGAAAACTTGAAACTTTGGTGTTTCCTTATATAAGCAAA
ATCCACTGATCTACATATGAAGCACTAATATGGAAATTGACGTATTGATTTTAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTAT
   
Sequence Name 74-K107937-0022-594-F06C-8409
GenBank Accession LN513087 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K107937-0022-594-F06C-8409
CATTGAATAATCCTAAAATCAATACGTCAATTTCCATATTAGTGCTTTATATGTAGATCA
GTGGATTTTGCTTATATAAGGAAACACCAAAGTTTCAAGTTTTCTGATCTAAAATTAGTA
ACTTTCTGATATTTTCTATAAGTAGAAAACTTGATGCACACTTGTGAGTTATAAGCAGTT
AACACTTTGGTCTATTTTCTACAAACCAAATAATTAGTAGTTTTAAGATTGAAGCAATCA
CAATGTCGGTGTGGATCAGGATAAACAAAGTTCTGACTTTTGGTAAATCCTTTTGCAGAA
GCATATGATGCGCTTGATCCAGAAGGCAACATTACAATGAAATGGGATGTTATGAGCTGG
ACTCCTGATGGCTATGTTGTAAGTTCAAGAACCTGAAGTAGAAAATATTAGGCCTTGTTT
ACCGGTTTTTGATTCATCTAAACTGTTTGTTTGTTCGATCTAGGCCGTGGTTACGATGTT
CAACTTCCAGAAATACAGACACATTCAATCTCCAGGATGGACATTAGGTTGGAAATGGGC
AAAGAAGGAAGTTATATGGAGTATGGTTGGAGCACAAACAACTGAACAAGGTAAC

Last Updated on 06.12.2018 16:11