Sequences from confirmation amplicon


Confirmation sequences for line 372G06 and hit At5g61120 are shown.
Line ID 372G06
Locus At5g61120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24583650
Primers used for PCR FQ60 - 8474, show primer details
   
Sequence Name 72-K033879-022-372-G06C-FQ60
GenBank Accession LN512667 [GenBank]
Sequencing primer gene specific primer, FQ60: 5'-CAAATCCAGTTCCTAAATGGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K033879-022-372-G06C-FQ60
AGTTAATNNTTCACCAGAGGAAGTAAAAAAACTCTGCATGTGGAGGCAGTACCAGTAAAC
CAACTTTTAGAGTACCAAGACCTGTTTCAGCTCGTCCTCCAATGGGTTTAACAAAACCAA
CTGCTGGCTTTCCAAGAGCTAGGAGTCTAAATTCTACAGTGGTGGCTCGCAAAACCAAAT
CAATTTACCTTCCACCTAAAAAGGTTGAGCCTCTTAGTCCTCGTACTCAACAGATTCGTC
CAGGGGTCATGAGACAAGCTTCTAAAGCACAAGCAGTAGGAGAAGGTGGAATCTTTTGCT
TCTCTCTCCGTTTTCTTAATCTATTTCGTTTCTAAAGGCCTAAGATCCCAAACTTCTTTT
ACTAGATGACTAAAAACATATCTTGCTGAGTTTTTTATTGGCTTTTCAAGGGTCAAAATC
AAAAGTTGGCGATGGACCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 16-K033879-022-372-G06C-8409
GenBank Accession LN493340 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K033879-022-372-G06C-8409
GTTGTACATTATTGGAGGACGAGCTGAAACAGGTCTTGGTACTCTAAAAGTTGGTTTACT
GGTACTGCCTCCACATGCAAGTTTTTTTACTTCCTCTGGTGAAATTAACTTCATCCTTGA
CGTTTCAGGAATTTTTTTCCATTTAGGAACTGGATTT

Last Updated on 06.12.2018 16:11