Sequences from confirmation amplicon


Confirmation sequences for line 835A02 and hit At5g61120 are shown.
Line ID 835A02
Locus At5g61120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24582671
Primers used for PCR NS50 - 8474, show primer details
   
Sequence Name 23-K102512-0022-835-A02C-8409
GenBank Accession LN496505 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K102512-0022-835-A02C-8409
ATTTACAATTGAAGGGTCTTTGATGAAGGGTCTTTGATTTACACATTGTCAACTACATAT
CCATCATCAAAATGTTGGGGAGTTTGATTCGATGTTCCTGGAAGAAGTGCTAAAGACTTT
TTCGTCAGGAGAAAAATCCCCCAAAGGAAGTTATTTGTTTTCTTCTGCATCTTGATGATA
ACTGCAAACACAAAACGAGTTTTATTCATATTTTTGAAGCCAATGATAGATCTGTAAGCA
TACATTAGGCTTACTCTGGGAGGACTTGTCCAGTAGTTTTGAAGAGAATATCAACAATGG
TGTGCCATTGATGTTAAATTTCATCATGGCATTGCGAATCCTCATGGCCTCAAAGATATG
ATCACGTTCCGCTGTAAACCTGCAATCGTCGTAAATTTTGTGATAACAATGAACAAGAAG
CTTTTCCTTTGTAGCCATAGTGGCTTGATTACCTTTTTGTGTTCTTGTCATCTGGGAAAA
TGTCATTCTCG
   
Sequence Name 42-K102513-0022-835-A02C-NS50
GenBank Accession LN504421 [GenBank]
Sequencing primer gene specific primer, NS50: 5'-GTCGAGATGTACATTTTCCCAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K102513-0022-835-A02C-NS50
GTATCAGCCATATGGCTACAAGGAAAAGCTTCTTGTTCATTGTTATCACAAAATTTACGA
CGATTGCAGGTTTACAGCGGAACGTGATCATATCTTTGAGGCCATGAGGATTCGCAATGC
CATGATGAAATTTAACATCAATGGCACACCATTGTTGATATTCTCTTCAAAACTACTGGA
CAAGTCCTCCCAGAGTAAGCCTAATGTATGCTTACAGATCTATCATTGGCTTCAAAAATA
TGAATAAAACTCGTTTTGTGTTTGCAGTTATCATCAAGATGCAGAAGAAAACAAATAACT
TCCTTTGGGGGATTTTTCTCCTGACGAAAAAGTCTTTAGCACTTCTTCCAGGAACATCGA
ATCAAACTCCCCAACATTTTGATGATGGATATGTAGTTGACAATGTGTAAATCAAAGACC
CTTCATCAAAGACCCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11