Sequences from confirmation amplicon


Confirmation sequences for line 654F09 and hit At5g63580 are shown.
Line ID 654F09
Locus At5g63580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25456407
Primers used for PCR 65xm - 8474, show primer details
   
Sequence Name 38-K119444-0022-654-F09C-65xm
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 65xm: 5'-AATTGCACGAGATACACGATAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K119444-0022-654-F09C-65xm
CGTCGCCCCTATAAGTTTTGCACTTGTTACAATTATTGTAGTAACAACTTTTGTTGGACC
ATCGTCACATAATCCAAAGTTAGTGGGTGGTAAAGTAAAAACTAGAGAAGCCACTTTTTC
ATTTTCTTTTTTAAAACGGATATTAATCGTCATTCACGTCATTGCATTTTTTCTATTTAT
AGTGACAATACATATCAATTTTCTAGACTAATTTTTTTGATACTTTATTCGGCGAAATAA
TTCGCTTACATCATTAATTAACACTTTTAAAATTAATGTAATTTTCTTTAAATATTTTGA
TGAAATTTGAATACAAAATATTAATATAGCACTTAAATACTTTTACCCGCCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAAGATTATT
   
Sequence Name 06-K119443-0022-654-F09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K119443-0022-654-F09C-8409
CATTGAATATATCCTGGCGGGTAAAAGTATTTAAGTGCTATATTAATATTTTGTATTCAA
ATTTCATCAAAATATTTAAAGAAAATTACATTAATTTTAAAAGTGTTAATTAATGATGTA
AGCGAATTATTTCGCCGAATAAAGTATCAAAAAAATTAGTCTAGAAAATTGATATGTATT
GTCACTATAAATAGAAAAAATGCAATGACGTGAATGACGATTAATATCCGTTTTAAAAAA
GAAAATGAAAAAGTGGCTTCTCTAGTTTTTACTTTACCACCCACTAACTTTGGATTATGT
GACGATGGTCCAACAAAAGTTGTTACTACAATAATTGTAACAAGTGCAAAACTTTATAGG
TGGCGACGTGGCGTCGTAAATATTTTGTATAAAGGTTATCGTGTATTCNGTGCAATTATT

Last Updated on 06.12.2018 16:11