Sequences from confirmation amplicon


Confirmation sequences for line 809C08 and hit At5g65660 are shown.
Line ID 809C08
Locus At5g65660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26246393
Primers used for PCR DA28 - 8474, show primer details
   
Sequence Name 42-K107550-0022-809-C08C-DA28
GenBank Accession LN504484 [GenBank]
Sequencing primer gene specific primer, DA28: 5'-TTGGGTTTGTAAGGATTAGACTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K107550-0022-809-C08C-DA28
GGCGGAGAGACGATGTTTGTCCCATGGTAACAACAAGAGAATATTCCGGAGAGGCTGAAA
ATGATGATAAGAAGCAGAGCTGTGCCAAGAGGGAATCCTAGTGACGGCCGAGAAGCATCC
ATGTGTGGTGGCGAGAAATCTTGAGTGTTCTCCATCTTCTTCTACGACGGTGGCGATATA
ATCGACGGCTGAGATTGTGAGCTGATGATTTTGTTCTCTCATGTGTTAGAGAGAGAAGGC
TAGAGAAGAGAAGAGAGCTTTGAGAGTTTGGTGAGAACAGGGGACGGGGAAACAAGAAGA
GATTATTGTTTTTATTTTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 34-K107549-0022-809-C08C-8409
GenBank Accession LN501442 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K107549-0022-809-C08C-8409
TCGGACTGAGCATTTACAATTGAATATAAAATAAAAACAATAATCTCTTCTTGTTTCCCC
GTCCCCTGTTCTCACCAAACTCTCAAAGCTCTCTTCTCTTCTCTAGCCTTCTCTCTCTAA
CACATGAGAGAACAAAATCATCAGCTCACAATCTCAGCCGTCGATTATATCGCCACCGTC
GTAGAAGAAGATGGAGAACACTCAAGATTTCTCGCCACCACACATGGATGCTTCTCGGCC
GTCACTAGGATTCCCTCTTGGCACAGCTCTGCTTCTTATCATCATTTTCAGCCTCTCCGG
AATATTCTCTTGTTGTTACCATTGGGACAAACATCGTTCTCTCCGCCGCTCTTTGGCCAA
TGGTCGTCCCTCCGCCGACATCGAGTCTAATCCTTACAAACCCA

Last Updated on 06.12.2018 16:11