Sequences from confirmation amplicon


Confirmation sequences for line 196D03 and hit At5g66340 are shown.
Line ID 196D03
Locus At5g66340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26502529
Primers used for PCR KT51 - 8474, show primer details
   
Sequence Name 87-K100817-0022-196-D03C-8409
GenBank Accession LN514727 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K100817-0022-196-D03C-8409
TTGAATATATCCTGAATTTGGATGATTTTGTCGTCCTTTTGTTTGTCGACAAAAAATTGG
TACCTTTTGTTTTACCAATCTCTGCATAGTCTTAGTGACCCGTTAGGATGATTTAGTGTC
CCACATGGCTTAGGTTTAGAGTTTTAGACACCTAACGCTAATGAGTGATCCTATCATATA
TGAACATTTTCGTCGTCTTCCATAGATTTAAAGTTAGTTAGAGCCAATGATTTGGAACCT
TTGCTTGCAACAGCTGCACATTCATTAACCCATCAACCTTGCTTCCTGTAAGGCTATAAT
TACTTCTTCTATCTATGTCATCAACCAAGACTAGTTTAGATTTTGACAACTTCTGGTCAA
ATGGGTACGTCACATATGGGAGAAAATGAAATAAATTGTAAAGTTTATTAGTCAATTTAT
GACATGTGAATTGGTAATATGTCAAATAATCCTAAATCAAGAGTTGTTCTGCTTTGGAAT
CTCAACAGAATCTAAAACTCATAACCTCTGAGTTATGGTGGATGGGGATAGGGCCAACCA
CACCTCANTGTACAGATGTTGTTGTCTTC
   
Sequence Name 87-K100829-0022-196-D03C-KT51
GenBank Accession LN514728 [GenBank]
Sequencing primer gene specific primer, KT51: 5'-AGACATATGCGTGAAGACAACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K100829-0022-196-D03C-KT51
CATCCCCATAACTCAGAGGTTATGAGTTTTAGATTCTGTTGAGATTCCAAAGCAGAACAA
CTCTTGATTTAGGATTATTTGACATATTACCAATTCACATGTCATAAATTGACTAATAAA
CTTTACAATTTATTTCATTTTCTCCCATATGTGACGTACCCATTTGACCAGAAGTTGTCA
AAATCTAAACTAGTCTTGGTTGATGACATAGATAGAAGAAGTAATTATAGCCTTACAGGA
AGCAAGGTTGATGGGTTAATGAATGTGCAGCTGTTGCAAGCAAAGGTTCCAAATCATTGG
CTCTAACTAACTTTAAATCTATGGAAGACGACGAANATGTTCATATATGATAGGATCACT
CATTAGCGTTAGGTGTCTAAAACTCTAAACCTAAGCCATGTGGGACACTAAATCATCCTA
ACGGGTCACTAAGACTATGCAGAGATTGGTAAAACAAAAGGTACCAATTTTTTGTCGACN
AACAAAAGGACGACNAAATCATCCNAATTCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11