Sequences from confirmation amplicon


Confirmation sequences for line 851F11 and hit At5g66870 are shown.
Line ID 851F11
Locus At5g66870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26706434
Primers used for PCR ME43 - 8474, show primer details
   
Sequence Name 77-K027642-022-851-F11C-8409
GenBank Accession LN513473 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K027642-022-851-F11C-8409
GATACATTTATTTCAATCTCTTCAACTTCATAGGGTCTCTCATCTAATGACTTACACGAA
GCTTCCTCGATGCATTTATTTTCACGATCCCTATTTCATTATTTTCAAGAAAATTTATTA
AAAGAGACTGAATCTTTTTATTTCTTTATAATCTTTTCAGGCTCTCAAGTCTGAAAAGAG
ACAAAAAAAATGGCGTCTTCAAGCTCTCCTTGCGCAGCTTGTAAATTCTTGAGAAGAAAA
TGCACACAAGAATGTGTATTCGCTCCATATTTCCCACCAGACCAACCTCAAAAATTCGCA
TTCGTTCACAAAGTCTTTGGAGCAAGTAACGTCGCTAAGCTTCTCAACGAGCTTGCCTCT
AACCAAAGAGAAGACGCAGTGAACTCTCTTTTCTACGAAGCTGAAGCTAGGTTACGTGAT
CCGGTTTACGGTTGCGTCGGTTTAATCTCAATCCTTCAACATCGTCTTAAACAAGTTAAC
CACGATCTTGAAAACGCTAAGAAAGAGCTCGCTACGTACGTTGGTCCTCAAGCTATGCTC
CCAATTCTTCAACCGCATTTCAGT
   
Sequence Name 78-K027642-022-851-F11C-ME43
GenBank Accession LN513618 [GenBank]
Sequencing primer gene specific primer, ME43: 5'-TTGAGGTGGTAGAGACATGAAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K027642-022-851-F11C-ME43
ACGTACGTAGCGAGCTCTTTCTTAGCGTTTTCAGATCGTGGTTAACTTGTTTAAGACGAT
GTTGAAGGATTGAGATTAAACCGACGCAACCGTAAACCGGATCACGTAACCTAGCTTCAG
CTTCGTAGAAAAGAGAGTTCACTGCGTCTTCTCTTTGGTTAGAGGCAAGCTCGTTGAGAA
GCTTAGCGACGTTACTTGCTCCAAAGACTTTGTGAACGAATGCGAATTTTTGAGGTTGGT
CTGGTGGGAAATATGGAGCGAATACACATTCTTGTGTGCATTTTCTTCTCAAGAATTTAC
AAGCTGCGCAAGGAGAGCTTGAAGACGCCATTTTTTTTGTCTCTTTTCAGACTTGAGAGC
CTGAAAAGATTATAAAGAAATAAAAAGATTCAGTCTCTTTTAATAAATTTTCTTGAAAAT
AATGAAATAGGGATCGTGAAAATAAATGCATCGAGGAAGCTTCGTGTAAGTCATTAGATG
AGAGACCCTATGAAGTTGAAGAGATTGTAAATAAATGTTATCGTGATTCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCANAAATGTAGATGTCCGCAGCGTTATTATAANATGAAAGTACATTTTGA
TAAAACGACNAATTACGATCCGTCGTATTTATAGGCGAAA

Last Updated on 06.12.2018 16:11