Sequences from confirmation amplicon


Confirmation sequences for line 068G07 and hit At5g67490 are shown.
Line ID 068G07
Locus At5g67490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26934999
Primers used for PCR 07ip - 8474, show primer details
   
Sequence Name 71-K113193-0022-068-G07C-07ip
GenBank Accession LN512588 [GenBank]
Sequencing primer gene specific primer, 07ip: 5'-CAGCTTAAACTCGATAGGATCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K113193-0022-068-G07C-07ip
TTTCATCTACGGATAACAAATTCGAAACACCAGAACTTCGAATCAATGAACAGGCATAAA
CGCACGCACTAATTGCGCGATTTCAACCGGAATCTTAACGATGAGAGAAGGAATATAGAA
GAAGAGCGATACGATGATAATTATACCTTTGGCTTTCTTGCCGATATCGGTGAAGAGTCC
TGGACCTTTGCTCATGGTTGATCGGAGATTGAGAGAGATCGGCAGGCGGTGAAGATATCA
AGATAGAAGAATTACGACTTGTTTTTCTCGTGGTAGACGAAAATAAATAGCGAAGCCAAT
ACTATATAACAGCCTGTGACTTTTCTTTAGTGACTAACCACTCACAGCCGTTGATGTGGC
ACAAAAAAACACATGACGCTNTATGAGATAATCGACAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 34-K113192-0022-068-G07C-8409
GenBank Accession LN501482 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K113192-0022-068-G07C-8409
CATTTACAATTGTCGATTATCTCATAAAGCGTCATGTGTTTTTTTGTGCCACATCAACGG
CTGTGAGTGGTTAGTCACTAAAGAAAAGTCACAGGCTGTTATATAGTATTGGCTTCGCTA
TTTATTTTCGTCTACCACGAGAAAAACAAGTCGTAATTCTTCTATCTTGATATCTTCACC
GCCTGCCGATCTCTCTCAATCTCCGATCAACCATGAGCAAAGGTCCAGGACTCTTCACCG
ATATCGGCAAGAAAGCCAAAGGTATAATTATCATCGTATCGCTCTTCTTCTATATTCCTT
CTCTCATCGTTAAGATTCCGGTTGAAATCGCGCAATTAGTGCGTGCGTTTATGCCTGTTC
ATTGATTCGAAGTTCTGGTGTTTCGAATTTGTTTATCCTGTAGATTGAAATGATTTTTAG
ATTTTGATTATATTTTGGGTGATCCTATCGAGTTTTAAGCTG
Primers used for PCR 84wa - 8474, show primer details
   
Sequence Name 27-K118126-0022-068-G07C-84wa
GenBank Accession LN498589 [GenBank]
Sequencing primer gene specific primer, 84wa: 5'-AATGATGATCCAAATAAAGGCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118126-0022-068-G07C-84wa
TTTTCTTCATTTTCTCCATTATTTTGCAATACATAAACACATAAAACGAATTTATTTCCA
TTTTTCCCAGAAATATATAAGAACAAGCTAAAACACAGGGATACATGAATCCTCTCAGAA
ATCAGAGCATCGACCACGTTGTTCCCAATCGCCGTACCGAGTCGGTTCAGGACCTCTAGG
CCCTCCAATCTCACCAGTATCTTCGTTCACAAACTCTCCACCTCCACCACCTTCTCCTTC
CTCTTCTTCTTCCTTCTGCATCTGCAGATTCTGTTGAAGATTCTGATCTGGCTTCACGTC
CCGGTTCAGATCCTGATTCGGAGATGGAGCTTGCGGCGGAGGAGGTGTACCGGAGCTTAG
AAACCGTGACACTGGCTCGAGGAATCTAGAGGAGACGGATCGGCTTGGAGCGATCAAACG
TACGATGTTGTTCGTCGCCATTGTTAGATCAGATGTGTCGGCCAAATCAAGAAAGAGATC
CGACCTTCACGGTTTGTGATAGTATAGGGTTCGATAGTCATTTAGGTTGGGCCATAATTG
GATAATAAGCCCAGTAGTATCTAGGCCCAATCATTTACATGTTTATAAATAAATTAAGAG
AATTAGAGAGTAAATTTGGCCCAGTATAATTATGTTTAACAAGCCCATCAGGAGCTAGGC
CTAACATGTTTATAAAACAAATAGGAGAGAATTAGAGGGTAAAGTTGACGTGGGCTGATT
TCGAACCCGTAACCTTTTTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 11-K118125-0022-068-G07C-8409
GenBank Accession LN491315 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118125-0022-068-G07C-8409
CATTTACATTGAATATATAAAAAGGTTACGGGTTCGAAATCAGCCCACGTCAACTTTACC
CTCTAATTCTCTCCTATTTGTTTTATAAACATGTTAGGCCTAGCTCCTGATGGGCTTGTT
AAACATAATTATACTGGGCCAAATTTACTCTCTAATTCTCTTAATTTATTTATAAACATG
TAAATGATTGGGCCTAGATACTACTGGGCTTATTATCCAATTATGGCCCAACCTAAATGA
CTATCGAACCCTATACTATCACAAACCGTGAAGGTCGGATCTCTTTCTTGATTTGGCCGA
CACATCTGATCTAACAATGGCGACGAACAACATCGTACGTTTGATCGCTCCAAGCCGATC
CGTCTCCTCTAGATTCCTCGAGCCAGTGTCACGGTTTCTAAGCTCCGGTACACCTCCTCC
GCCGCAAGCTCCATCTCCGAATCAGGATCTGAACCGGGACGTGAAGCCAGATCAGAATCT
TCAACAGAATCTGCAGATGCAGAAGGAAGAAGAAGAGGAAGGAGAAGGTGGTGGAGGTGG
AGAGTTTGTGAACGAAGATACTGGTGAGATTGGAGGGCCTAGAGGTCCTGAACCGACTCG
GTACGGCGATTGGGAACAACGTGGTCGATGCTCTGATTTCTGAGAGGATTCATGTATCCC
TGTGTTTTAGCTTGTTCTTATATATTTCTGGGAAAAATGGAAATAAATTCGTTTTATGTG
TTTATGTATTTGCAAAATAATGGAGAAAATGGAAGAAAATGTAGTAGAACTTCAAATTCG
TTGCCTTTA

Last Updated on 06.12.2018 16:11