Sequences from confirmation amplicon


Confirmation sequences for line 857H02 and hit At5g67490 are shown.
Line ID 857H02
Locus At5g67490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26934733
Primers used for PCR JF25 - 8474, show primer details
   
Sequence Name 83-K102437-0022-857-H02C-8409
GenBank Accession LN514294 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K102437-0022-857-H02C-8409
TCGGACTGAGCCTTTACAATTGAATATATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGG
AGCTTGCATGTTGGTTTCTACGCAGAACTGAGCCGGTTAGGCAGATAATTTCCATTGAGA
ACTGAGCCATGTGCACCTTCCCCCCAACACGGTGAGCGACGGGGCAACGGAGTGATCCAC
ATGGGATAGTATAGAACAACGGACCGACCTTCACGGTTTGTGATAGTATAGGGTTCGATA
GTCATTTAGGTTGGGCCATAATTGGATAATAAGCCCAGTAGTATCTAGGCCCAATCATTT
ACATGTTTATAAATAAATTAAGAGAATTAGAGAGTAAATTTGGCCCAGTATAATTATGTT
TAACAAGCCCATCAGGAGCTAGGCCTAACATGTTTATAAAACAAATAGGAGAGAATTAGA
GGGTAAAGTTGACGTGGGCTGATTTCGAACCCGTAACCTTTTTATATATTAGACCTCATT
ACCAAAAAGCGATTCGATTATCTCATAAAGCGTCATGTGTTTTTTTGTGCCACATCAACG
GCTGTGAGTGGTTAGTCACTAAAGAAAAGTCACAGGCTGTTATATAGTATTGGCTTCGCT
ATTTATTTTCGTCTACCACGAGAAAAACAAGTCGTAATTCTTCTATCTTGA
   
Sequence Name 83-K102438-0022-857-H02C-JF25
GenBank Accession LN514295 [GenBank]
Sequencing primer gene specific primer, JF25: 5'-AGGCGGTGAAGATATCAAGATAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K102438-0022-857-H02C-JF25
AAATAATAGCGAAGCCATACTATATAACAGCCTGTGACTTTTCTTTAGTGACTAACCACT
CACAGCCGTTGATGTGGCACAAAAAAACACATGACGCTTTATGAGATAATCGAATCGCTT
TTTGGTAATGAGGTCTAATATATAAAAAGGTTACGGGTTCGAAATCAGCCCACGTCAACT
TTACCCTCTAATTCTCTCCTATTTGTTTTATAAACATGTTAGGCCTAGCTCCTGATGGGC
TTGTTAAACATAATTATACTGGGCCAAATTTACTCTCTAATTCTCTTAATTTATTTATAA
ACATGTAAATGATTGGGCCTAGATACTACTGGGCTTATTATCCAATTATGGCCCAACCTA
AATGACTATCGAACCCTATACTATCACAAACCGTGAAGGTCGGTCCGTTGTTCTATACTA
TCCCATGTGGATCACTCCGTTGCCCCGTCGCTCACCGTGTTGGGGGGAAGGTGCACATGG
CTCAGTTCTCAATGGAAATTATCTGCCTAACCGGCTCAGTTCTGCGTAGAAACCAACATG
CAAGCTCCACCGGGTGCAAAGCGGCAGCGGCGGCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11