Sequences from confirmation amplicon


Confirmation sequences for line 197C04 and hit F2K11 are shown.
Line ID 197C04
Locus F2K11 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23573233
Primers used for PCR KT50 - 8474, show primer details
   
Sequence Name 53-K100815-0022-197-C04C-8409
GenBank Accession LN508079 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K100815-0022-197-C04C-8409
CATTTACAATTGAATATATCATACTCATATTTACAAGCAAACCGTGAAACCCTACTTTCT
TCTCTCCGTGACCGTTCCTCCCTTGGAACCTATTCCAATGCCACAATCGGTCTTAGCCCC
GACACAGTCTATGGCATGTTTCTCTGCCGAGGAGACCTCACCAAAACATCATGTTCGGAC
TGTGTCAAAACCGCAACCCTAGAAATCACTAAAAATAATAACTGCACTTCCAGAAAAACT
GCTCTAATATTCTACGAGGAGTGTATGGTTCGATACTCCAATGTTTCCTTCTTCACGCTC
GTAGAACTAGACGGGCCTTACGTCGCTAAATACTCTCTCGCCACTTTTCCTACTTCTCTT
TTTAGTTCTTTCCAACAAACGTTATCCAATAAGGTAGAGCAACTAATTATCCTCATAGCA
TCAAAGTCTTCTTTGTCTGCTTCTACGCCTTATTACGTGAAGGATATATCACGTGTAAAT
GAATTAGAGGGCTCGTATACATTAGACACGGTGG
   
Sequence Name 53-K100827-0022-197-C04C-KT50
GenBank Accession LN508081 [GenBank]
Sequencing primer gene specific primer, KT50: 5'-GACTACACTGAACCACCGTGTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K100827-0022-197-C04C-KT50
TATATCCTTCACGTAATAAGGCGTAGAAGCAGACAAAGAAGACTTTGATGCTATGAGGAT
AATTAGTTGCTCTACCTTATTGGATAACGTTTGTTGGAAAGAACTAAAAAGAGAAGTAGG
AAAAGTGGCGAGAGAGTATTTAGCGACGTAAGGCCCGTCTAGTTCTACGAGCGTGAAGAA
GGAAACATTGGAGTATCGAACCATACACTCCTCGTAGAATATTAGAGCAGTTTTTCTGGA
AGTGCAGTTATTATTTTTAGTGATTTCTAGGGTTGCGGTTTTGACACAGTCCGAACATGA
TGTTTTGGTGAGGTCTCCTCGGCAGAGAAACATGCCATAGACTGTGTCGGGGCTAAGACC
GATGGTGGCATTGGAATAGGTTCCAAGGGAGGAACGGTCACGGAGAGAAGAAAGTAGGGT
TTCACGGTTTGCTTTGTAAATATGAGTATGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
Primers used for PCR 18lf - 8474, show primer details
   
Sequence Name 76-K113920-0022-197-C04C-8409
GenBank Accession LN513439 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K113920-0022-197-C04C-8409
GAATATATCATACTCATATTTACAAAGCAAACCGTGAAACCCTACTTTCTTCTCTCCGTG
ACCGTTCCTCCCTTGGAACCTATTCCAATGCCACAATCGGTCTTAGCCCCGACACAGTCT
ATGGCATGTTTCTCTGCCGAGGAGACCTCACCAAAACATCATGTTCGGACTGTGTCAAAA
CCGCAACCCTAGAAATCACTAAAAATAATAACTGCACTTCCAGAAAAACTGCTCTAATAT
TCTACGAGGAGTGTATGGTTCGATACTCCAATGTTTCCTTCTTCACGCTCGTAGAACTAG
ACGGGCCTTACGTCGCTAAATACTCTCTCGCCACTTTTCCTACTTCTCTTTTTAGTTCTT
TCCAACAAACGTTATCCAATAAGGTAGAGCAACTAATTATCCTCATAGCATCAAAGTCTT
CTTTGTCTGCTTCTACGCCTTATTACGTGAAGGATATATCACGTGTAAATGAATTAGAGG
GCTCGTATACATTAGACACGGTGGTTCAGTGTAGTCCTGATCTTGATCCAGCAAATTGCG
GCGTCTGTTTGAGACTTGTGGTCAAAGGTCTCTCAGGCTGTTGTACCAA
   
Sequence Name 66-K113919-0022-197-C04C-18lf
GenBank Accession LN511660 [GenBank]
Sequencing primer gene specific primer, 18lf: 5'-AGCAAAACGAGCATTGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K113919-0022-197-C04C-18lf
CGACGCCGCAATTTGCTGGATCAGATCAGGACTACACTGAACCACCGTGTCTAATGTATA
CGAGCCCTCTAATTCATTTACACGTGATATATCCTTCACGTAATAAGGCGTAGAAGCAGA
CAAAGAAGACTTTGATGCTATGAGGATAATTAGTTGCTCTACCTTATTGGATAACGTTTG
TTGGAAAGAACTAAAAAGAGAAGTAGGAAAAGTGGCGAGAGAGTATTTAGCGACGTAAGG
CCCGTCTAGTTCTACGAGCGTGAAGAAGGAAACATTGGAGTATCGAACCATACACTCCTC
GTAGAATATTAGAGCAGTTTTTCTGGAAGTGCAGTTATTATTTTTAGTGATTTCTAGGGT
TGCGGTTTTGACACAGTCCGAACATGATGTTTTGGTGAGGTCTCCTCGGCAGAGAAACAT
GCCATAGACTGTGTCGGGGCTAAGACCGATTGTGGCATTGGAATAGGTTCCAAGGGAGGA
ACGGTCACGGAGAGAAGAAAGTAGGGTTTCACGGTTTGCTTTGTAAATATGAGTATGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TATCCGCAGCGTTAT

Last Updated on 06.12.2018 16:11