Sequences from confirmation amplicon


Confirmation sequences for line 758H10 and hit MBG8 are shown.
Line ID 758H10
Locus MBG8 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22323709
Primers used for PCR 07vp - 8474, show primer details
   
Sequence Name 69-K117997-0022-758-H10C-07vp
GenBank Accession LN512279 [GenBank]
Sequencing primer gene specific primer, 07vp: 5'-GGCCAAGATCAAGAACTAACAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K117997-0022-758-H10C-07vp
GTATCATAATTTTCTAACTTATAATTGTTGCAAATCATCTGTTAACTATTCAATGCAATT
TGATTCCTTACTTAATTTTGTGATCATGGATACGTTCATGTATTGTTTTTCAACCTCTTA
CAAAGTCTTCACTAGAATAATTAAACTACCTAGACGCAGTCATATAAAGAAACTCTTACA
AAGCCCAAACTAGTGCCGTTTTGATATATATAAAAAGTGTTCTCCAGAAAGCTTTCTTGC
AAGTTTCGAGAAGGGATCTTCGAAATTGAAGTTGGTCCAAGCAGCAATCTCACAATAATC
CACCTTCCTCCCTATACCGTGCGGGTCCAAACTTTCTAAACTTTTCTTTGATGAACTAAA
AAATTGTGTCTTTTTGTTAATGTAAAAAAAAAAAGCAGTTCTCCAAAAAAAAGTGTTTAA
CAATGTAAAGCCTAAGCAATGGCTTCACTTGGTTGTCCTCCGGAACGGCCCAACAGTATG
CAACTCCAAAGTCTTAATACATGCAAGACCAACCTAAAACTAAGCAATCATTGGATATGT
TGAGACTGATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGAGTATGA
TGGTCAATATGGAGAAAAAAAAAGAGTAATTACCATTTTTTTTTCAATTCAAAAATGTAA
ATGTCC
   
Sequence Name 29-K117996-0022-758-H10C-8409
GenBank Accession LN499468 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K117996-0022-758-H10C-8409
ATCAGTCTCAACATATCCAATGATTGCTTAGTTTTAGGTTGGTCTTGCATGTATTAAGAC
TTTGGAGTTGCATACTGTTGGGCCGTTCCGGAGGACAACCAAGTGAAGCCATCGCTTAGG
CTTTACATTGTTAAACACTTCTTTTTGGAGAACTGCTTTTTTTTTTTCCTTTAACAAAAA
GACCCATTTTTTTAGTTCATCAAAAAAAATTTTAAAAAGTTTGGACCCCCACGGTATAGG
GAGGAAGGGGGATTATTGGGAAATTGCTGCTTGGACCAACTTCAATTTCAAAAATCCCTT
CTCAAAACTTGCAAAAAAGTTTTCTGGAAAACCCTTTTTATATTTTTCAAAACGGCCCTA
GTTGGGGCTTTGAAAAATTTTCTTTATATGACTGCGTCTAGGTAGTTTAATTATTCTAGG
GAAAACTTTGTAAAAGGTTGAAAAACAATACTTGAACGTTTCCTTGATCCCAAAATTAAG
TAGGGAATCAAATTGCTTTGAATAGTTAACAAATGATTTGCAACATTTTTAAGTTAAGAA
AATTAAGGATCCTTTTCCAATCAAAGATCAAAAAACTCTGTTCTTTGTTAGTTCTTGATC
TGGG

Last Updated on 06.12.2018 16:11