Sequences from confirmation amplicon


Confirmation sequences for line 174A07 and hit T14G11 are shown.
Line ID 174A07
Locus T14G11 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14419194
Primers used for PCR MK22 - 8474, show primer details
   
Sequence Name 04-K032004-022-174-A07C-MK22
GenBank Accession LN487801 [GenBank]
Sequencing primer gene specific primer, MK22: 5'-AGAACAAAATCAACCTCCTTTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K032004-022-174-A07C-MK22
CCAGTCCCCACTTGATTTTAGACTTTTTAGTGTTTGTGGTTGCATCTTTTTTGGTAAATC
TGGCTGTCTCGCCAATTTTGCTTTGCTCTAGTGATCCAATCAAAATAATTTCTGATTCTC
TTTCTTTTAGAAATAATTTTTCCGAACATAAAAGTTTAAGAAATTTAACTTTAATAACGA
AATTCATTATGTATAAAATAATTCTCGTGAAATTGTAAGAACTAAGAAGCATATCAAATG
CTTATCTTTGATCAAAAAAAAATAAAAAATGCTTATCTTTTGTTGTATCAGATAGAAAGT
GAAAATATTTAAAGAAGTGTACAAACAAGGACATAATCTTTTTTCTAGTGTGTCAAGTAC
TGACTCAAATTAAAGAACACAGCGAATTTCACGGCTTAGGGCTTCGGGTACTTATATAGT
ACTAATGATTATTTTCCAAGATCCTATATGGCTTCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 03-K032004-022-174-A07C-8409
GenBank Accession LN487366 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K032004-022-174-A07C-8409
TTACAATTGAATAATCCTGAAGCCATATAGGATCTTGGAAAATAATCATTAGTACTATAT
AAGTACCCGAAGCCCTAAGCCGTGAAATTCGCTGTGTTCTTTAATTTGAGTCAGTACTTG
ACACACTAGAAAAAAGATTATGTCCTTGTTTGTACACTTCTTTAAATATTTTCACTTTCT
ATCTGATACAACAAAAGATAAGCATTTTTTATTTTTTTTTGATCAAAGATAAGCATTTGA
TATGCTTCTTAGTTCTTACAATTTCACGAGAATTATTTTATACATAATGAATTTCGTTAT
TAAAGTTAAATTTCTTAAACTTTTATGTTCGGAAAAATTATTTCTAAAAGAAAGAGAATC
AGAAATTATTTTGATTGGATCACTAGAGCAAAGCAAAATTGGCGAGACAGCCAGATTTAC
CAAAAAAGATGCAACCACAAACACTAAAAAGTCTAAAATCAAGTGGGTGACTGGGTGGTA
GTCTAGTGGCATTTGCGCTTGGGTTTTTTTCAAAGGAGGTGAATTTTGTT

Last Updated on 06.12.2018 16:11