Sequences from confirmation amplicon


Confirmation sequences for line 590G04 and hit T5J8 are shown.
Line ID 590G04
Locus T5J8 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1300824
Primers used for PCR 60xm - 8474, show primer details
   
Sequence Name 48-K119407-0022-590-G04C-60xm
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 60xm: 5'-TATACATGAAAACGTAACCTTCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K119407-0022-590-G04C-60xm
TTTGTCAGAAAACAAAGAGACTATAAATAATAAGTTGAGAATAGACAATTGATGTATATT
TGAAATATGTATAACATACGTAGTCCAGAATATGAAGTTTTTGTTTGTTAATATTGAGTG
TTAACTCATTACATTAAACAAGTTTAATTAAGATCATTAGACGTATCACTAGGCTAAAGA
TACATCATCTTTTATTAGAATCTATTATTCAAACTAATATGATTATAATGTTATTTATAT
GTTTTACGTATGCAACATAGAATACTACTACACAGTTGCTGATTAATATTCCAAAACAAT
AAAGGAAGCTTTTGTTTTCCGGACCACATGGGAATGTGAATGGAAAATATGCTCTTGTTT
TACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCG
   
Sequence Name 08-K119406-0022-590-G04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K119406-0022-590-G04C-8409
TTACAATTGAATATATCCTGTAAAACAAGAGCATATTTTCCATTCACATTCCCATGTGGT
CCGGAAAACAAAAGCTTCCTTTATTGTTTTGGAATATTAATCAGCAACTGTGTAGTAGTA
TTCTATGTTGCATACGTAAAACATATAAATAACATTATAATCATATTAGTTTGAATAATA
GATTCTAATAAAAGATGATGTATCTTTAGCCTAGTGATACGTCTAATGATCTTAATTAAA
CTTGTTTAATGTAATGAGTTAACACTCAATATTAACAAACAAAAACTTCATATTCTGGAC
TACGTATGTTATACATATTTCAAATATACATCAATTGTCTATTCTCAACTTATTATTTAT
AGTCTCTTTGTTTTCTTGACAAATTATTGGTCATATTTTTCTTTCACATGGTTCCGAAGG
TTACGTTTTCCATGTATA

Last Updated on 06.12.2018 16:11