Sequences from confirmation amplicon


Confirmation sequences for line 002A10 and hit At3g14310 are shown.
Line ID 002A10
Locus At3g14310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4774933
Primers used for PCR TH24 - 8474, show primer details
   
Sequence Name 36-K103676-0022-002-A10C-TH24
GenBank Accession LN502196 [GenBank]
Sequencing primer gene specific primer, TH24: 5'-CAAGGCTTTACGAACTTGCTTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K103676-0022-002-A10C-TH24
GTCTCCTGGTTGGAATGGCGGAGCTAATTAGTGTTTTGAGATCTCCGGCGTGTTCCCGGA
GAGTTTTTTTGGTTGGATACAAATGGAGATCTTCCACCGTTTCATGGAGCTCGTCCAGCG
TCTCATCAATAGTCTCCAAACAGTCATGAAGCGCCGTCTTCTCGCGCGGCGTCAGTCCTT
TCCTCTTCTTGATCAGCTTCTTCACGGTGAAGTAATTGTGCTCCACGGCGGTTATTGTTA
GGTTCACCGATGCCTCGATGACATCTTTTTGCGACGTTAGCTCGACGCCGCCGGCAGTTA
CCACGGCAGAGATACAGAGCTCAGGGTAACGCGTGGAGCTGCATGAGGATCTTAGCACGG
CGTGAGACAATTTGATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGT
   
Sequence Name 52-K103677-0022-002-A10C-8409
GenBank Accession LN507789 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K103677-0022-002-A10C-8409
TCTCACGCCGTGCTAGATCCTCATGCAGCTCCACGCGTTACCCTGAGCTCTGTATCTCTG
CCGTGGTAACTGCCGGCGGCGTCGAGCTAACGTCGCAAAAAGATGTCATCGAGGCATCGG
TGAACCTAACAATAACCGCCGTGGAGCACAATTACTTCACCGTGAAGAAGCTGATCAAGA
AGAGGAAAGGACTGACGCCGCGCGAGAAGACGGCGCTTCATGACTGTTTGGAGACTATTG
ATGAGACGCTGGACGAGCTCCATGAAACGGTGGAAGATCTCCATTTGTATCCAACCAAAA
AAACTCTCCGGGAACACGCCGGAGATCTCAAAACACTAATTAGCTCCGCCATTACCAACC
AGGAGACTTGTCTCGACGGCTTCTCTCATGACGACGCCAATNAGCAAGTTCGTAAAGCCT

Last Updated on 06.12.2018 16:11