Sequences from confirmation amplicon


Confirmation sequences for line 002G02 and hit At4g35190 are shown.
Line ID 002G02
Locus At4g35190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16747583
Primers used for PCR MC89 - 8474, show primer details
   
Sequence Name 42-K102431-0022-002-G02C-8409
GenBank Accession LN504417 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K102431-0022-002-G02C-8409
ATTTACAATTGAATAATCCTGATTTTGTATATTGACCATCATATTCACTTACAGGCTTGT
CGTGAATTCCAAGTTGTGCCCATGCTATTACTTCCAATAACTCCTCCAGTGTTCCATACC
CACCTGATAACCATTTGCAAGAACAGATCAATTTTAAAGTCAATTACAAAATGAGTCTTG
TTTTGGAAGAAAGATCAAACTGTACCTAGTTTCAAATTTGTACCTAACGAATGACAAACA
TTTTTCAAGCTTTACAAAGTATCTAAGTTAGTTAGTTATTATGTTGTACCTGGTAAAGCA
ATGAAACAATCCGAGTGGCGTGCCATTTCGGCTTTTCTTTCATGCATATCCGCCACAGCT
ATTACCTCACCATATGTTTCTCCGGTTATCTGTTATAGAATCACTTTTCAGG
   
Sequence Name 42-K102432-0022-002-G02C-MC89
GenBank Accession LN504418 [GenBank]
Sequencing primer gene specific primer, MC89: 5'-AAAAGAGTTGTCCCTGAAAAGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K102432-0022-002-G02C-MC89
GAGGTATAGCTGTGGCGGATATGCATGAAAGAAAAGCCGAAATGGCACGCCACTCGGATT
GTTTCATTGCTTTACCAGGTACAACATAATAACTAACTAACTTAGATACTTTGTAAAGCT
TGAAAAATGTTTGTCATTCGTTAGGTACAAATTTGAAACTAGGTACAGTTTGATCTTTCT
TCCAAAACAAGACTCATTTTGTAATTGACTTTAAAATTGATCTGTTCTTGCAAATGGTTA
TCAGGTGGGTATGGAACACTGGAGGAGTTATTGGAAGTAATAGCATGGGCACAACTTGGA
ATTCACGACAAGCCTGTAAGTGAATATGATGGTCAATATACAAAATCAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
CATTCA

Last Updated on 06.12.2018 16:11