Sequences from confirmation amplicon


Confirmation sequences for line 003B09 and hit At5g17040 are shown.
Line ID 003B09
Locus At5g17040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5605421
Primers used for PCR HV17 - 8474, show primer details
   
Sequence Name 13-K031934-022-003-B09C-8409
GenBank Accession LN491922 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K031934-022-003-B09C-8409
GCCATTTCGCTCTCGGAAGGAAGCTCCTTTGAGAATTTCAAAGGTTTGTTGGACGAAGTT
ATGAAAGTTTGAAGCAAATAGACTTTGGATAAATAAACAAATAAACATGTTCTGTTTTGG
AATGAAAATGAAAAGACCTTTTTTGTAAAAAAAAAGTGAAAGTGAGAATCTTTATCCTTT
GTTTTTATTTTTTATTTTTTTCTGTTATGGTAACTTTGTAATTAATATATACATATCTAA
ACAAATTGTATGTCGGTAGATATATGTTTTGAGAAAAAAATATCTATCGTGTAGACTAGT
CTCCATATAAAAAAATATCTATACGTCTGTCGATCTAGGGGCTTACTTTAGAACATTGAA
TGAAAGACAGATGAGACTTGATGAGAAAACTCACCATATAATATAAGAACCACAAATTCT
ACCTACCAAAACAATATAGCAGGGACATAAACAAAGGACAAAATAAAGGTATACTTAAAT
TTGATTAAGAGAGAAAGAGTCTATACTTGCTCGGTGGCTCATCTACGAACTCTCTTTAGC
GTACGAACTCTCTTTAGCGTACTTCTATTTAATGTAACTACGCACATTACTCTCTCTTTA
GCATACTTCTATTTGATTAATGTAACTACGCACATTTTTCTCTCTTTAGCGTACTTCTAT
TTAATGTAACTACGCACATTATTCTCTCC
   
Sequence Name 14-K031934-022-003-B09C-HV17
GenBank Accession LN492394 [GenBank]
Sequencing primer gene specific primer, HV17: 5'-GGCAATGCTAAGGAGAGAATAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K031934-022-003-B09C-HV17
CGCTAAGAGAGAAAATGTGCGTAGTTACATTAATCAAATAGAAGTATGCTAAAGAGAGAG
TAATGTGCGTAGTTACATTAAATAGAAGTACGCTAAAGAGAGTTCGTACGCTAAAGAGAG
TTCGTAGATGAGCCACCGAGCAAGTATAGACTCTTTCTCTCTTAATCAAATTTAAGTATA
CCTTTATTTTGTCCTTTGTTTATGTCCCTGCTATATTGTTTTGGTAGGTAGAATTTGTGG
TTCTTATATTATATGGTGAGTTTTCTCATCAAGTCTCATCTGTCTTTCATTCAATGTTCT
AAAGTAAGCCCCTAGATCGACAGACGTATAGATATTTTTTTATATGGAGACTAGTCTACA
CGATAGATATTTTTTTCTCAAAACATATATCTACCGACATACAATTTGTTTAGATATGTA
TATATTAATTACAAAGTTACCATAACAGAAAAAAATAAAAAATAAAAACAAAGGATAAAG
ATTCTCACTTTCACTTTTTTTTTACAAAAAAGGTCTTTTCATTTTCATTCCAAAACAGAA
CATGTTTATTTGTTTATTTATCCAAAGTCTATTTGCTTCAAACTTTCATAACTTCGTCCA
ACAAACCTTTGAAATTCTCAAAGGAGCTTCCTTCCGTAGAGCGAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATNTCGACGTGTC

Last Updated on 06.12.2018 16:11