Sequences from confirmation amplicon


Confirmation sequences for line 004F03 and hit At3g05150 are shown.
Line ID 004F03
Locus At3g05150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1442910
Primers used for PCR 11bk - 8474, show primer details
   
Sequence Name 65-K107287-0022-004-F03C-11bk
GenBank Accession LN511413 [GenBank]
Sequencing primer gene specific primer, 11bk: 5'-TTTTTGTTCCATTTTTGGAGTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K107287-0022-004-F03C-11bk
TTCTATACTCGATCGAACAGTCGTTCTCACAGCACTAGGCGCAACATTGCTAATTGATCG
GCTTGGAAGAAGGCCACTTCTTATGGTATGGTTCTTGTTTCAGTTTCCAATAAATCCTAG
AATACCGGTTTAGAGTATTCGGTCTGACCATGCGGTACATTGCAGGCTTCAGCTGTTGGG
ATGCTGATTGGATGTTTGCTCATCGGAAATTCATTTCTTTTGAAGGTCAATCATATGAAA
AATAAAATTAAAAAATTAAAGCGTATAATGTCAGGAAAAATATAGGACGTTTAGGTTAAC
CGGTTTGATTTTGGTTTTGACAGGCTCATGGTTTAGCACTTGACATCATTCCGGCTCTGG
CAGTTAGTGGTGTCTTGGTAAACATCTATAAACCTAATTTTGGTTTCAATATATATGGTT
TGGTCTTGGTTTTTTCCTCGGTTGACCCAAAACCCAATTTCCAGGTCTACATCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGGTTATTA
   
Sequence Name 49-K107286-0022-004-F03C-8409
GenBank Accession LN506859 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K107286-0022-004-F03C-8409
ATTTACATTGAATTATCCTGATGTAGACCTGGAAATTGGGTTTTGGGTCAACCGAGGAAA
AAACCAAGACCAAACCATATATATTGAAACCAAAATTAGGTTTATAGATGTTTACCAAGA
CACCACTAACTGCCAGAGCCGGAATGATGTCAAGTGCTAAACCATGAGCCTGTCAAAACC
AAAATCAAACCGGTTAACCTAAACGTCCTATATTTTTCCTGACATTATACGCTTTAATTT
TTTAATTTTATTTTTCATATGATTGACCTTCAAAAGAAATGAATTTCCGATGAGCAAACA
TCCAATCAGCATCCCAACAGCTGAAGCCTGCAATGTACCGCATGGTCAGACCGAATACTC
TAAACCGGTATTCTAGGATTTATTGGAAACTGAAACAAGAACCATACCATAAGAAGTGGC
CTTCTTCCAAGCCGATCAATTAGCAATGTTGCGCCTAGTGCTGTGAGAACGACTTGTTCG
ATCGAGTATAGAATGCTTCCAAGAGTTGGGGATGCTCCTGGTACTCCAAAAATGGAACAA
AA

Last Updated on 06.12.2018 16:11