Sequences from confirmation amplicon


Confirmation sequences for line 004F08 and hit At4g31510 are shown.
Line ID 004F08
Locus At4g31510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15276781
Primers used for PCR 02cy - 8474, show primer details
   
Sequence Name 11-K109236-0022-004-F08C-02cy
GenBank Accession LN491155 [GenBank]
Sequencing primer gene specific primer, 02cy: 5'-TCTGGTAGGTTCTACGTTTAGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K109236-0022-004-F08C-02cy
CGGCGTCTCTGTCTGCCGGATTGGTTTGAGGAGATGTGGTAGATCTCCTCCGCCGGAGAG
TTCGTCGTCCGTTGGAGAAACCAGCGAAAACGAGGAAGATGAAGACGACGCCGTTTCGTC
ATCACAAGGAAGATGGCTCAATTCGTTTAGTTCATCTTTGGAAGATTCTCTTCCGATTAA
GTACGTTTCTTTATTAATTATCTTCTAATTATTCTGTTATCTACACCTGAATATATTCGA
TCGATTTATTTTATTACAAGAATAAAGTAAATCATTAGATTCTCTTATGTTCTTGGGTTT
GATTTTATTGATGAATCTGTAAGATGTTGAAATTGTTTCTTACTTATTAGATTCTCAGTT
TAAATGTTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 03-K109235-0022-004-F08C-8409
GenBank Accession LN487524 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K109235-0022-004-F08C-8409
CATTTACATTGAATATACATTTAAACTGAGAATCTAATAAGTAAGAAACAATTTCAACAT
CTTACAGATTCATCAATAAAATCAAACCCAAGAACATAAGAGAATCTAATGATTTACTTT
ATTCTTGTAATAAAATAAATCGATCGAATATATTCAGGTGTAGATAACAGAATAATTAGA
AGATAATTAATAAAGAAACGTACTTAATCGGAAGAGAATCTTCCAAAGATGAACTAAACG
AATTGAGCCATCTTCCTTGTGATGACGAAACGGCGTCGTCTTCATCTTCCTCGTTTTCGC
TGGTTTCTCCAACGGACGACGAACTCTCCGGCGGAGGAGATCTACCACATCTCCTCAAAC
CAATCCGGCTAGACAGAGACGCCGGTACGGCCTGATCATGCGTCGTGACGCTGCTACGAT
CCCTAAACGTAGAACCTACCAGA

Last Updated on 06.12.2018 16:11