Sequences from confirmation amplicon


Confirmation sequences for line 004G05 and hit At3g06330 are shown.
Line ID 004G05
Locus At3g06330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1918190
Primers used for PCR 12bk - 8474, show primer details
   
Sequence Name 66-K107287-0022-004-G05C-12bk
GenBank Accession LN511623 [GenBank]
Sequencing primer gene specific primer, 12bk: 5'-TTTGTGGTTGTCAAAAGATATGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K107287-0022-004-G05C-12bk
TCGGGCATGGACAGGACCTTGAATATCTTTCTGCAACAAAGATTAAATCTTTACTTGTAT
ATATTGAGCAAGGAAGGACTATATTGTAAGAAAGAAATAACTCACATTAGGCGGGTCACC
AGGCACCCCAGCTGAAAGTTGTGTAGAAACCACAGGAGACAGTTTTGCAGCTTGTCTACT
AGGAAGCGATAAGCATCTTTTCCAATATGGAGAAGTTGTGGAACCAGTAGCATTAGGCTT
TTTAGCGGTTTCCATGAGACCAGGAGATAGTAAGGAGCTTCTCTCGCTTTCGGGTTGAGA
AATAACTTTCTTTTTCAAGCTTAAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 50-K107286-0022-004-G05C-8409
GenBank Accession LN507186 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K107286-0022-004-G05C-8409
TTACATTGAATTTAGCTTGAAAAAGAAAGTTATTTCTCAACCCGAAAGCGAGAGAAGCTC
CTTACTATCTCCTGGTCTCATGGAAACCGCTAAAAAGCCTAATGCTACTGGTTCCACAAC
TTCTCCATATTGGAAAAGATGCTTATCGCTTCCTAGTAGACAAGCTGCAAAACTGTCTCC
TGTGGTTTCTACACAACTTTCAGCTGGGGTGCCTGGTGACCCGCCTAATGTGAGTTATTT
CTTTCTTACAATATAGTCCTTCCTTGCTCAATATATACAAGTAAAGATTTAATCTTTGTT
GCAGAAAGATTATTCAAGGTCCTTGTCCATGCCCGGAAGAAACAAAGTTATTGTGCGATC
CATATCTTTTGACAACCACAA

Last Updated on 06.12.2018 16:11