Sequences from confirmation amplicon


Confirmation sequences for line 005D04 and hit At1g15690 are shown.
Line ID 005D04
Locus At1g15690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5400954
Primers used for PCR YN05 - 8474, show primer details
   
Sequence Name 45-K024725-022-005-D04C-8409
GenBank Accession LN505395 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K024725-022-005-D04C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCACCAGTATATCAGTTG
AAAGAAATGGTTACAAATCCGTCATTATTCCAATCTTTGCTATTGCTATCAGTATATTCG
TTAGCTTCAGCTTTGCTGCTATGTATGGTGTTGCTGTTGCTGCTCTTGGTATGCTCAGTA
CCATTGCCACTGGTTTGGCAATTGATGCTTATGGTCCCATCAGTGACAATGCTGGTGGTA
TTGCTGAAATGGCTGGAATGAGCCACCGCATCCGTGAAAGAACTGATGCTCTTGATGCCG
CTGGAAACACCACTGCTGCTATTGGAAAGGTATGGAGCCTTCTTAACCTCTTTCTGCAAT
CCGATGAATTAATAAAAGAGCTTGCTTGCTTGAACCATTCCTCAGGTTGCCTTTGCTTGT
GTTGTATTTTGACATGTTATTCTGGTTTGTTGTGTACAGGGATTTGCCATTGGCTCTGCT
GCCCTAGTCTCCTTGGCTCTCTTTGGTGCCTTTGTGAGCCGTGCAGGGATCCACACCGTA
GATGTTTTGACCCCTAA
   
Sequence Name 46-K024725-022-005-D04C-YN05
GenBank Accession LN505740 [GenBank]
Sequencing primer gene specific primer, YN05: 5'-CCAATGATAACTTTAGGGGTCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K024725-022-005-D04C-YN05
CGGTGTGGATCCCTGCACGGCTCACAAAGGCACCAAAGAGAGCCAAGGAGACTAGGGCAG
CAGAGCCAATGGCAAATCCCTGTACACAACAAACCAGAATAACATGTCAAAATACAACAC
AAGCAAAGGCAACCTGAGGAATGGTTCAAGCAAGCAAGCTCTTTTATTAATTCATCGGAT
TGCAGAAAGAGGTTAAGAAGGCTCCATACCTTTCCAATAGCAGCAGTGGTGTTTCCAGCG
GCATCAAGAGCATCAGTTCTTTCACGGATGCGGTGGCTCATTCCAGCCATTTCAGCAATA
CCACCAGCATTGTCACTGATGGGACCATAAGCATCAATTGCCAAACCAGTGGCAATGGTA
CTGAGCATACCAAGAGCAGCAACAGCAACACCATACATAGCAGCAAAGCTGAAGCTAACG
AATATACTGATAGCAATAGCAAAGATTGGAATAATGACGGATTTGTAACCATTTCTTTCA
ACTGATATACTGGTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGA

Last Updated on 06.12.2018 16:11