Sequences from confirmation amplicon


Confirmation sequences for line 007E10 and hit At5g26780 are shown.
Line ID 007E10
Locus At5g26780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9418692
Primers used for PCR 01ai - 8474, show primer details
   
Sequence Name 38-K106635-0022-007-E10C-8409
GenBank Accession LN502992 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K106635-0022-007-E10C-8409
GCCTTTACAATTGAATATATCAACAAAATTTAGTGAGAATGATCAAATTCACATATATAG
AAGCTTTGATTTATTGATTTGGGGGATGATGTTACGAGTTTTTTCAGGGATTTGAACTTA
TACCGTCAGAGAATTTCACTTCTCTATCTGTGATGCAAGCCGTTGGTTCTGTTATGACTA
ATAAGTATAGTGAAGGATACCCTGGAGCTAGATACTATGGAGGAAATGAGTATGTATTTG
TTATACTTACAATGGTTTACTTTGTTGTTAGTATATGATGCTTAAGGTTGTTTGCATCTT
ATTGACTAGGTACATTGACATGGCTGAGACATTATGTCAAAAACGCGCATTAGAAGCTTT
TCAGTTAGATCCTTCTAAATGGGGAGGTGTGCTAATTACATACTTTCGTTGTGAATTGTG
ATTAAAGAAATCCTACTAGAAACCAAATTTACTAATGGAGACACTTGTTCATTTTGTAGT
CAATGTGCAGTCTTTATCAGGATCACCAGCTAATTTCCAAGTTTACACTGCATTGTTGAA
GCCTCATGAAAGAATCATGGCACTTGATTTACCTCATGGTGGACATCTTTCTCATGGTTA
TCAGT
   
Sequence Name 54-K106636-0022-007-E10C-01ai
GenBank Accession LN508490 [GenBank]
Sequencing primer gene specific primer, 01ai: 5'-ACCCAAGTACCTGATAACCATGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K106636-0022-007-E10C-01ai
GATTCTTTCATGAGGCTTCAACAATGCAGTGTAAACTTGGAAATTAGCTGGTGATCCTGA
TAAAGACTGCACATTGACTACAAAATGAACAAGTGTCTCCATTAGTAAATTTGGTTTCTA
GTAGGATTTCTTTAATCACAATTCACAACGAAAGTATGTAATTAGCACACCTCCCCATTT
AGAAGGATCTAACTGAAAAGCTTCTAATGCGCGTTTTTGACATAATGTCTCAGCCATGTC
AATGTACCTAGTCAATAAGATGCAAACAACCTTAAGCATCATATACTAACAACAAAGTAA
ACCATTGTAAGTATAACAAATACATACTCATTTCCTCCATAGTATCTAGCTCCAGGGTAT
CCTTCACTATACTTATTAGTCATAACAGAACCAACGGCTTGCATCACAGATAGAGAAGTG
AAATTCTCTGACGGTATAAGTTCAAATCCCTGAAAAAACTCGTAACATCATCCCCCAAAT
CAATAAATCAAAGCTTCTATATATGTGAATTTGATCATTCTCACTAAATTTTGTTGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCC

Last Updated on 06.12.2018 16:11