Sequences from confirmation amplicon


Confirmation sequences for line 008H11 and hit At3g01310 are shown.
Line ID 008H11
Locus At3g01310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:98723
Primers used for PCR MG03 - 8474, show primer details
   
Sequence Name 75-K031454-022-008-H11C-MG03
GenBank Accession LN513169 [GenBank]
Sequencing primer gene specific primer, MG03: 5'-AAGAAAACTTCAACGGGATAGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K031454-022-008-H11C-MG03
TGTTACTATAGACATGTTTTTGGTACCTTGTACGGGGAACTAACATTCTTGTGGCATCTA
ATAGATCTTGCAACTGGACGGCACTCTTAAGTTTGGTCTGCAAAAGGGGAGAAATTGACA
CAGTATAAGCAAATTAATCAAGACAAGCTGAACTTGAAGATATAAAGACTTGAGCCTCTC
ACCTCAGCTCTTGGCTTTCCACCATTGTACTTCAACATTAGGTTTAATAGTTTTTCCTCT
GTAACTTTTAGTTTCACTTTCTGCTTGGGAGTTCTATCGCCACTGACATGTAAAACAAAG
TTGAACACTTCTCAGATCCTGACTAAACAGAGAGAAGGATAGGAAGAACAGGTGATTATA
TGCTACAACATACTGTCGAACAACAGCAATGACACAACGTAGCTCTTCTGACTGCCCAAA
AGTGCCGATGATGCCACTTCCCTGGCGAGTTAGACCTTCATTAGATTGTACAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTCACTCCCC
   
Sequence Name 67-K031454-022-008-H11C-8409
GenBank Accession LN511732 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K031454-022-008-H11C-8409
TTTACAATTGAAATATCCTGTACATCTAATGAAGGTCTAACTCGCCAGGGAAGTGGCATC
ATCGGCACTTTTGGGCAGTCAGAAGAGCTACGTTGTGTCATTGCTGTTGTTCGACAGTAT
GTTGTAGCATATAATCACCTGTTCTTCCTATCCTTCTCTCTGTTTAGTCAGGATCTGAGA
AGTGTTCAACTTTGTTTTACATGTCAGTGGCGATAGAACTCCCAAGCAGAAAGTGAAACT
AAAAGTTACAGAGGAAAAACTATTAAACCTAATGTTGAAGTACAATGGTGGAAAGCCAAG
AGCTGAGGTGAGAGGCTCAAGTCTTTATATCTTCAAGTTCAGCTTGTCTTGATTAATTTG
CTTATACTGTGTCAATTTCTCCCCTTTTGCAGACCAAACTTAAGAGTGCCGTCCAGTTGC
AAGATCTATTAGATGCCACAAGAATGTTAGTTCCCCGTACAAGGTACCAAAAACATGTCT
TAATAGTAACATGTCTTAGTCCAAATCTTTCGAAGGGGATTTTGTCTATCCCGTTGAAGT
TTTCT

Last Updated on 06.12.2018 16:11