Sequences from confirmation amplicon


Confirmation sequences for line 009D11 and hit At1g08520 are shown.
Line ID 009D11
Locus At1g08520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2696415
Primers used for PCR 02lu - 8474, show primer details
   
Sequence Name 14-K114404-0022-009-D11C-8409
GenBank Accession LN492605 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K114404-0022-009-D11C-8409
TGCATCAGGAAAGGATGACGAGACAAAAGATAGAGAAGCAAAAGTAAGCTGATAAGGTTT
GATACAGTAGAAAATACTATCTCTTAACTTCTTCTTCTTCTTCTTCTTCTTCTCCTATCT
TTGAAAATGGCGATGACTCCGGTCGCGTCATCATCTCCAGTTTCAACCTGCAGACTCTTT
CGCTGCAATCTCCTCCCTGATCTCTTACCTAAGCCTCTGTTTCTCTCCCTCCCCAAACGA
AACAGAATTGCCTCGTGCCGCTTCACTGTACGTGCCTCCGCGAATGCTACCGTCGAATCC
CCTAACGGTGTCCCTGCCTCCACATCAGATACGGATACGGAGACGGATACCACCTCCTAT
GGCCGACAGTTTTTCCCTTTGGCCGCAGTTGTTGGCCAGGTCAGAGAATTTGCAATTTGA
TTCTGCAGGTTGCATAATTCTACTCAATTTGATTTAGTTGGAGTTGGGTATAGGAAAAAG
CTTCAGTTTTTCATCAGTGAACTGTGTGTTATGGGACTTTTACATTTGTTTGTTCAACTT
GTTAGATGCCGAAAGCCTCACAGAGGATGTAATTTGTTTGCTTTAACTAAATGTTGTTTG
TAGGAAGGCATAAAAACTGC
   
Sequence Name 36-K114405-0022-009-D11C-02lu
GenBank Accession LN502319 [GenBank]
Sequencing primer gene specific primer, 02lu: 5'-AGCAGTTTTTATGCCTTCCTACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K114405-0022-009-D11C-02lu
TCCTCTGTGAGGCTTTCGGCATCTAACAAGTTGAACAAACAAATGTAAAAGTCCCATAAC
ACACAGTTCACTGATGAAAAACTGAAGCTTTTTCCTATACCCAACTCCAACTAAATCAAA
TTGAGTAGAATTATGCAACCTGCAGAATCAAATTGCAAATTCTCTGACCTGGCCAACAAC
TGCGGCCAAAGGGAAAAACTGTCGGCCATAGGAGGTGGTATCCGTCTCCGTATCCGTATC
TGATGTGGAGGCAGGGACACCGTTAGGGGATTCGACGGTAGCATTCGCGGAGGCACGTAC
AGTGAAGCGGCACGAGGCAATTCTGTTTCGTTTGGGGAGGGAGAGAAACAGAGGCTTAGG
TAAGAGATCAGGGAGGAGATTGCAGCGAAAGAGTCTGCAGGTTGAAACTGGAGATGATGA
CGCGACCGGAGTCATCGCCATTTTCAAAGATAGGAGAAGAAGAAGAAGAAGAAGAAGAAG
TTAAGAGATAGTATTTTCTACTGTATCAAACCTTATCAGCTTACTTTTGCTTCTCTATCT
NTTGTCTCGTCATCCTTTCCTGATTGCAGCAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCANAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11