Sequences from confirmation amplicon


Confirmation sequences for line 009E11 and hit At3g44850 are shown.
Line ID 009E11
Locus At3g44850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16375965
Primers used for PCR 91mm - 8474, show primer details
   
Sequence Name 86-K114351-0022-009-E11C-91mm
GenBank Accession LN514683 [GenBank]
Sequencing primer gene specific primer, 91mm: 5'-CTAAAAGTTGAATCCTTAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K114351-0022-009-E11C-91mm
ATCGTTAGATCTCTTAGTTTTTTAGGCTAGAAAACCAATTTACCAGCATAAAAATGATTC
CTTTATTAGGTTTCTTAGTATATAAGACTCTTTGAGTGTAATGTGCTTGATCAAGTTGTT
TGACTTGTTGTAGAGAAATGCTAAAGGTTGAATCTTTAGGCTTAGATCAGTAGTTCTTTA
GTTTCATGTGTGAAGTTTTGAAAGTTCTTTTTTGTGTAGCGTTATGTAGCTTTGAAAGTT
CAGAAGAGTGCTCAACATTATACAGAGGCAGCAATGGATGAGATAAAGATTTTGAAACAG
ATAGCTGAAGGTGACTCGGGTGATAAGAAGTGTGTTGTAAAGCTACTGGATCATTTCAAG
CATACGGGTCCTAATGGGAAGCATGTTTGTATGGTTTTCGAGTATTTGGGAGATAACCTT
TTGTCGGTTATTAAGTACAGTGATTACCGAGGGGTTCCTCTTCACATGGTTAAAGAGCTT
TGTTTTCATATTTTGGTTGGGTTGGATTATCTTCATCGTGAGCTTTCTATTATTCATACT
GATTTAAAGCCGGAGAATGTTCAATTGACTCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATT
   
Sequence Name 38-K114350-0022-009-E11C-8409
GenBank Accession LN503066 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K114350-0022-009-E11C-8409
CATTTACATTGAATATATCCTGAGTCAATTGAACATTCTCCGGCTTTAAATCAGTATGAA
TAATAGAAAGCTCACGATGAAGATAATCCAACCCAACCAAAATATGAAAACAAAGCTCTT
TAACCATGTGAAGAGGAACCCCTCGGTAATCACTGTACTTAATAACCGACAAAAGGTTAT
CTCCCAAATACTCGAAAACCATACAAACATGCTTCCCATTAGGACCCGTATGCTTGAAAT
GATCCAGTAGCTTTACAACACACTTCTTATCACCCGAGTCACCTTCAGCTATCTGTTTCA
AAATCTTTATCTCATCCATTGCTGCCTCTGTATAATGTTGAGCACTCTTCTGAACTTTCA
AAGCTACATAACGCTACACAAAAAAGAACTTTCAAAACTTCACACATGAAACTAAAGAAC
TACTGATCTAAGCCTAAAGATTCAACCTTTAGCATTTCTCTACAACAAGTCAAACAACTT
GATCAAGCACATTACACTCAAAGAGTCTTATATACTAAGAAACCTAATAAAGGAATCATT
TTTATGCTGGTAAATTGGTTTTCTAGCCTAAAAAACTAAGAGATCTAACGATCTATGAAT
AACCCATCCCGGGTAATCGGTAATCTAAGACTAA

Last Updated on 06.12.2018 16:11