Sequences from confirmation amplicon


Confirmation sequences for line 010A10 and hit At1g09240 are shown.
Line ID 010A10
Locus At1g09240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2985149
Primers used for PCR IW21 - 8474, show primer details
   
Sequence Name 48-K016268-022-010-A10C-8409
GenBank Accession LN506356 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K016268-022-010-A10C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATCTCTTGGACTCTGTCACACATCTT
GGTGACATCGATGTTAGGGTTTGGTGGTATGCATGTGGAAACGAGCTGCTTGAAGAGAAT
GTTGACATCTTCGGATGGTTTTAGACTCTCAAGCTTTGAGATCTTTTCGTAGAGATCGCA
TATTGTTTGCACCAATTGTTCGTCTTGGCAACCCATGTCGACACTATGAGGTTATTGATT
TTTCTCTTTCTCTTTGGAGAATAATCGTGTGTTCTCGGGTGGTGTGAACTGTTTGGACTG
GAGGGATGGATATTTATTGGCGTGGGGTTGGGGATCTACATCAACTAGAAAAAGAATCTA
ATTATTTTAACATTTCATTATTCAAATATACTATATAACGTACACTTGCGTTTTTGTTTC
TTTTTTTGTTTAACTAATTAAGAATAGATCCTTATATTTAGGTTTTGAAGTAAAAGATTT
ATTTAAGTCAAGATATTAGTTTAATGTTTACAGTATTGGTTTAGTTTCTATGATTTCTGA
TTTAGGTTTAAGGTTTTAGTTTAGGTTTAGAGTGTATGTTTTCGATTATAGAAATTTCAT
TCCTAATTGCTATAAAATTTACAATCATTTTCAAAATTATCCTGGTTATGAAAAATGAC
   
Sequence Name 52-K016268-022-010-A10C-IW21
GenBank Accession LN507659 [GenBank]
Sequencing primer gene specific primer, IW21: 5'-TCCATGTTCACACAAGTCATTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K016268-022-010-A10C-iw21
CCAGGATAATTTTGAAAATGATTGTAAATTTTATAGCAATTAGGAATGAAATTTCTATAA
TCGAAAACATACACTCTAAACCTAAACTAAAACCTTAAACCTAAATCAGAAATCATAGAA
ACTAAACCAATACTGTAAACATTAAACTAATATCTTGACTTAAATAAATCTTTTACTTCA
AAACCTAAATATAAGGATCTATTCTTAATTAGTTAAACAAAAAAAGAAACAAAAACGCAA
GTGTACGTTATATAGTATATTTGAATAATGAAATGTTAAAATAATTAGATTCTTTTTCTA
GTTGATGTAGATCCCCAACCCCACGCCAATAAATATCCATCCCTCCAGTCCAAACAGTTC
ACACCACCCGAGAACACACGATTATTCTCCAAAGAGAAAGAGAAAAATCAATAACCTCAT
AGTGTCGACATGGGTTGCCAAGACGAACAATTGGTGCAAACAATATGCGATCTCTACGAA
AAGATCTCAAAGCTTGAGAGTCTAAAACCATCCGAAGATGTCAACATTCTCTTCAAGCAG
CTCGTTTCCACATGCATACCACCAAACCCTAACATCGATGTCACCAAGATGTGTGACAGA
GTCCAAGAGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGA
Primers used for PCR FM61 - 3144, show primer details
   
Sequence Name 45-K029300-022-010-A10C-3144
GenBank Accession LN505414 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K029300-022-010-A10C-3144
CTTCGCAGACCCTTCCTCTATATAAGGATAGTTCATTTCATTTGGAGTGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGACAGGATATA
TTGGCGGGTAAACCTAAGAGAAAAGAGCGTTTATTAGAATAATCGGATATTTAGATTCGA
TTTCCCGGACATGTAGATTTGTGGTCTAGCCGAAGGTCACTTAGAAAACCATTTCTCTTC
GATCTTGACCTCTTACCAAGACAACCCACTTCATCATTTAAACATTTTCCCTTATTACAA
CAACTATTTGAAACTCGGAAAGCTCGAGTTCGACCTCCTCGAACAAAACCTAAATGGCTT
TGTCCCAAAGAGTGTGGCTTTCATTGGATCTGGTCCTCTTCCTCTCACTTCCATCGTTCT
TGCTTCATTCCATCTCAAAGACACAATCTTTCACAACTTTGACATCGACCCATCAGCGAA
CTCACTCGCTTCTCTTCTGGTTTCCTCTGATCCAGACATCTCTCAACGCATGTTCTTCCA
CACCGTTGATATAATGGACGTGACAGAGAGCTTAAAGAGCTTTGATGTCGTGTTTCTAGC
TGCTCTTGTTGGAATGAA
   
Sequence Name 46-K029300-022-010-A10C-FM61
GenBank Accession LN505758 [GenBank]
Sequencing primer gene specific primer, FM61: 5'-ACTTTCTCCTCTTTGTTCATTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K029300-022-010-A10C-FM61
TCAAGCTCTTTAGCTCTCTGTCACGTCCATTATATCAACGGTGTGGAAGAACATGCGTTG
AGAGATGTCTGGATCAGAGGAAACCAGAAGAGAAGCGAGTGAGTTCGCTGATGGGTCGAT
GTCAAAGTTGTGAAAGATTGTGTCTTTGAGATGGAATGAAGCAAGAACGATGGAAGTGAG
AGGAAGAGGACCAGATCCAATGAAAGCCACACTCTTTGGGACAAAGCCATTTAGGTTTTG
TTCGAGGAGGTCGAACTCGAGCTTTCCGAGTTTCAAATAGTTGTTGTAATAAGGGAAAAT
GTTTAAATGATGAAGTGGGTTGTCTTGGTAAGAGGTCAAGATCGAAGAGAAATGGTTTTC
TAAGTGACCTTCGGCTAGACCACAAATCTACATGTCCGGGAAATCGAATCTAAATATCCG
ATTATTCTAATAAACGCTCTTTTCTCTTAGGTTTACCCGCCAATATATCCTGTCAAACAC
TGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCC
AAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCG
TCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGA
ACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTG

Last Updated on 06.12.2018 16:11