Sequences from confirmation amplicon


Confirmation sequences for line 010D10 and hit At2g17190 are shown.
Line ID 010D10
Locus At2g17190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7480240
Primers used for PCR KU76 - 8474, show primer details
   
Sequence Name 07-K103353-0022-010-D10C-KU76
GenBank Accession LN489232 [GenBank]
Sequencing primer gene specific primer, KU76: 5'-TCAATGGCTGTTGTTGATATGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K103353-0022-010-D10C-KU76
GAGCCTCAATGTTTCCTTTCTCTGTGGACCATTTTATATATAGGTTTGCAGGCTGATCAC
ACCGTTCATATGGTTCGAGGTTTTGTGCCTTCTTCTCCTTCTGCTCCCGCTGCAAATGCT
GGAAACCAAACCACTGCTCCTCAAGCGGTTGGTTCAAATGATAGCTCAAACCTCGGAGGA
GGTGAATCTTTGTTCCCTGGCCTTGGATTTAATCCTTTGGGTGGTGGAAATGCTATGGCT
GGATTATTTGGATCTGGTCTTCCTGATCTTGAGCAGGCACAGCAACAACTAGCTCAGAAC
CCTAACATGATTAGAGAGATGATGAATACTCCAGCCATCCAGAATCTTATGAACAACCCA
GAATTTATGAGGAGTATGATTATGAATAACCCTCAAATGCGTGAGCTTGTAGATCGCAAT
CCTGAGCTTGGTCATGTGCTCAATGACCCAAGCATCCTTCGTCAAACTCTAGAAGCGGCG
AGAAACCCAGAGCTTATGCGTAGAAACCAACATGCAAGCTCCACCGGGTGCAAAGCGGCA
GCGGCGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCA
   
Sequence Name 23-K103354-0022-010-D10C-8409
GenBank Accession LN496517 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K103354-0022-010-D10C-8409
ATTTACAATTGAATATATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGGAGCTTGCATGT
TGGTTTCTACGCATAAGCTCTGGGTTTCTCGCCGCTTCTAGAGTTTGACGAAGGATGCTT
GGGTCATTGAGCACATGACCAAGCTCAGGATTGCGATCTACAAGCTCACGCATTTGAGGG
TTATTCATAATCATACTCCTCATAAATTCTGGGTTGTTCATAAGATTCTGGATGGCTGGA
GTATTCATCATCTCTCTAATCATGTTAGGGTTCTGAGCTAGTTGTTGCTGTGCCTGCTCA
AGATCAGGAAGACCAGATCCAAATAATCCAGCCATAGCATTTCCACCACCCAAAGGATTA
AATCCAAGGCCAGGGAACAAAGATTCACCTCCTCCGAGGTTTGAGCTATCATTTGAACCA
ACCGCTTGAGGAGCAGTGGTTTGGTTTCCAGCATTTGCAGCGGGAGCAGAAGGAGAAGAA
GGCACAAAACCTCGAACCATATGAACGGTGTGATCAGCCTGCAAACCTATATATAAAATG
GTCCACAGAGAAAGGAAACATTGAGGCTCATGATGCAAAACAATAAAACGTGGAAACTAT
CCAACACAATCATATCAAC
Primers used for PCR 63lf - 3144, show primer details
   
Sequence Name 74-K113840-0022-010-D10C-63lf
GenBank Accession LN513115 [GenBank]
Sequencing primer gene specific primer, 63lf: 5'-AGCCCCACAATATGTTCTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K113840-0022-010-D10C-63lf
TTACTAGAACAACAGCCAAGAAACTTATTCATAGACAAACTAGTCATAACAACAAACTGT
GAGAACATGAACTCACCAATTATTACTATAATCAATACTATTCCTGACCAGTGTGGTAAA
CATAAACTTTCACTTCTTAAAAACAAGCCACTCTTGCTTTTATATTTAATATTTCAACCT
TAAACTACAAGTAAAATCCCATATGAAATTAAGAGGATGAGAATACAGTTTCTTACCAGC
CGTGGCACCCCAAGGATTAGGAAGTGGATTTGCATTAGGTGTACCCGTTTCAGCATTCGG
TGCCGAAATATTATTAGAAGTATCGCTTCCTTGTGTAGTAACCCCCTGGTTTCCCAGAAG
AGCAGCAAATGGATTAGAGCTCGTATTGTTTCCAGCATTCTCGGACATAGTGGTAGCATT
CATTAACGGTTCCTGGACATTTTCATACATACGCCTAAGCATGTTAAACACTGATAGTTT
AAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAAT
GAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTT
ACGTCAGAGGAGATATCA
   
Sequence Name 66-K113841-0022-010-D10C-35St
GenBank Accession LN511658 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K113841-0022-010-D10C-35St
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAAC
ATGCTTAGGCGTATGTATGAAAATGTCCAGGAACCGTTAATGAATGCTACCACTATGTCC
GAGAATGCTGGAAACAATACGAGCTCTAATCCATTTGCTGCTCTTCTGGGAAACCAGGGG
GTTACTACACAAGGAAGCGATACTTCTAATAATATTTCGGCACCGAATGCTGAAACGGGT
ACACCTAATGCAAATCCACTTCCTAATCCTTGGGGTGCCACGGCTGGTAAGAAACTGTAT
TCTCATCCTCTTAATTTCATATGGGATTTTACTTGTAGTTTAAGGTTGAAATATTAAATA
TAAAAGCAAGAGTGGCTTGTTTTTAAGAAGTGAAAGTTTATGTTTACCACACTGGTCAGG
AATAGTATTGATTATAGTAATAATTGGTGAGTTCATGTTCTCACAGTTTGTTGTTATGAC
TAGTTTGTCTATGAATAAGTTTCTTGGCTGTTTGTTTCTAGTTTAACTGTTACTCGTAAT
GCACTAAAGATTTTAGAACATTGGTGGGGGC
Primers used for PCR KU77 - 3144, show primer details
   
Sequence Name 39-K118366-0022-010-D10C-KU77
GenBank Accession LN503514 [GenBank]
Sequencing primer gene specific primer, KU77: 5'-TCCTGACCAGTGTGGTAAACATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K118366-0022-010-D10C-KU77
CAGCCACTCTTGCTTTTATTTAATATTTCAACCTTAAACTACAAGTAAAATCCCATATGA
AATTAAGAGGATGAGAATACAGTTTCTTACCAGCCGTGGCACCCCAAGGATTAGGAAGTG
GATTTGCATTAGGTGTACCCGTTTCAGCATTCGGTGCCGAAATATTATTAGAAGTATCGC
TTCCTTGTGTAGTAACCCCCTGGTTTCCCAGAAGAGCAGCAAATGGATTAGAGCTCGTAT
TGTTTCCAGCATTCTCGGACATAGTGGTAGCATTCATTAACGGTTCCTGGACATTTTCAT
ACATACGCCTAAGCATGTTAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCT
GATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTC
TTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATAAAANCAATC
CAC
   
Sequence Name 60-K118365-0022-010-D10C-35St
GenBank Accession LN510369 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K118365-0022-010-D10C-35St
ATCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGA
CAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAA
CATGCTTAGGCGTATGTATGAAAATGTCCAGGAACCGTTAATGAATGCTACCACTATGTC
CGAGAATGCTGGAAACAATACGAGCTCTAATCCATTTGCTGCTCTTCTGGGAAACCAGGG
GGTTACTACACAAGGAAGCGATACTTCTAATAATATTTCGGCACCGAATGCTGAAACGGG
TACACCTAATGCAAATCCACTTCCTAATCCTTGGGGTGCCACGGCTGGTAAGAAACTGTA
TTCTCATCCTCTTAATTTCATATGGGATTTTACTTGTAGTTTAAGGTTGAAATATTAAAT
ATAAAAGCAAGAGTGGCTTGTTTTTAAGAAGTGAAAGTTTATGTTTACCACACTGNGTCA
GG

Last Updated on 06.12.2018 16:11