Sequences from confirmation amplicon


Confirmation sequences for line 011A11 and hit At5g50420 are shown.
Line ID 011A11
Locus At5g50420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20531696
Primers used for PCR 01hd - 8474, show primer details
   
Sequence Name 39-K112477-0022-011-A11C-01hd
GenBank Accession LN503401 [GenBank]
Sequencing primer gene specific primer, 01hd: 5'-GAAAGCAAGATGAAAAAGCCATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K112477-0022-011-A11C-01hd
GTGCGATGAATTCTTTCCCAGAAGGTCTGGATGAATCTCTGTGCGGTCAACAAGATCAGC
TTACTAGGTTCGATTAGTGTCTTACACTTGTGGTTAATAGGTCCACCAGGTTGCATCACC
CAATCTTGCTCCATATCAGCATAGAAGACATCCCCGATTGCAATCACATCATCATCAGAC
TTAAACTTCATCTGGACGTCTTGCACAGTTCTCTTGCTCGGCTTCTTTATGTCTTCACTC
CAAGGAGCCTCAAGCTTCCCATCAATGGAAATCCCCAAACCTTTCAGCTTTTTAATATGA
AACTGTGAAAACGACAATGGATCAGCAATGAGTATATGGAGAAAAAAAAAGAGTAAATGG
CTTGAATATTAGAATAATATGGAGATTACCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATA
   
Sequence Name 16-K112476-0022-011-A11C-8409
GenBank Accession LN493491 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K112476-0022-011-A11C-8409
GCATTTCATTGGNAATCTCCATATTATTCTAATATTCAAGCCATTTACTCTTTTTTTTTC
TCCATATACTCATTGCTGATCCATTGTCGTTTTCACAGTTTCATATTAAAAAGCTGAAAG
GTTTGGGGATTTCCATTGATGGGAAGCTTGAGGCTCCTTGGAGTGAAGACATAAAGAAGC
CGAGCAAGAGAACTGTGCAAGACGTCCAGATGAAGTTTAAGTCTGATGATGATGTGATTG
CAATCGGGGATGTCTTCTATGCTGATATGGAGCAAGATTGGGTGATGCAACCTGGTGGAC
CTATTAACCACAAGTGTAAGACACTAATCGAACCTAGTAAGCTGATCTTGTTGACCGCAC
AGAGATTCATCCAGACCTTCTTGGGAAAGAATTTCATCGCACTTCATTTCCGCAGACATG
GGTTCCTCAAATTTTGGTATGGCTTTTTCATCTTGCTTT

Last Updated on 06.12.2018 16:11