Sequences from confirmation amplicon


Confirmation sequences for line 012B02 and hit At3g48140 are shown.
Line ID 012B02
Locus At3g48140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17779263
Primers used for PCR 54we - 8474, show primer details
   
Sequence Name 75-K118544-0022-012-B02C-54we
GenBank Accession LN513304 [GenBank]
Sequencing primer gene specific primer, 54we: 5'-TGATTTTTGCTACTTCAGACCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K118544-0022-012-B02C-54we
ATCATCATCTTATTTTTTGAAATGGAACAAAACAACTTAAATAGCTAGAACAGAGATGTT
TTTGATTCTAAAGTTTCTTGCATTAGGGTTTGTTTTTTGATTCAGTGTATTTTAAAGGGT
TTAGATTTTGGATGCAATGTGTTCTCTAAGGGTTTAAACATGAATAGATTACTGCCTAAC
AAACATTCTTGACTTTATCTATTTTTAGAGTTCTAAACAGAGATGTTTGGACTCTTGAAG
TTTCTTGCCACTGAAATTAGGGTTTGTTTTTGGATTCAGAGTGTGGTTTCTAATGGGTTT
TGTTTGTTGAATCCTTTCTTCAGATGCACCAAGGAGAACAGGGCTGCTGGAATTTTGGAT
AACCATGCAGAGGGAGAGAAGTATAAGGAAAATTTCCTGAGGAAGTTTGTTCGCAACAAG
AAACCTGAAATCATGCCTGGACTCAACAAGTTCTTCACTGATCCTACATACTGAAACCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 55-K118543-0022-012-B02C-8409
GenBank Accession LN508943 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K118543-0022-012-B02C-8409
AGGCATGATTTCAGGTTTCTTGTTGCGAACAAACTTCCTCAGGAAATTTTCCTTATACTT
CTCTCCCTCTGCATGGTTATCCAAAATTCCAGCAGCCCTGTTCTCCTTGGTGCATCTGAA
GAAAGGATTCAACAAACAAAACCCATTAGAAACCACACTCTGAATCCAAAAACAAACCCT
AATTTCAGTGGCAAGAAACTTCAAGAGTCCAAACATCTCTGTTTAGAACTCTAAAAATAG
ATAAAGTCAAGAATGTTTGTTAGGCAGTAATCTATTCATGTTTAAACCCTTAGAGAACAC
ATTGCATCCAAAATCTAAACCCTTTAAAATACACTGAATCAAAAAACAAACCCTAATGCA
AGAAACTTTAGAATCAAAAACATCTCTGTTCTAGCTATTTAAGTTGTTTTGTTCCATTTC
AAAAAATAAAGATGATGATCTAAAAACCTGATTCCATAAGATCAATTGTGTAAAAGGGTC
TG

Last Updated on 06.12.2018 16:11