Sequences from confirmation amplicon


Confirmation sequences for line 012B07 and hit At3g16340 are shown.
Line ID 012B07
Locus At3g16340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5544055
Primers used for PCR KC14 - 8474, show primer details
   
Sequence Name 18-K035535-022-012-B07C-8409
GenBank Accession LN494213 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K035535-022-012-B07C-8409
AGCCATTTAAATTGAATATAGCCATTTACATTTGGGGTATTAACAGCTTTGATGGGAGTT
AGTGGAGCTGGTAAGACCACTTTAATGGATGTTTTAGCTGGTAGAAAAACCGGTGGTTAC
ATCGAAGGAGACATCAGAATCTCTGGTTTCCCTAAAAGACAAGAAACCTTTGCAAGAATC
TCTGGTTATTGTGAACAGAACGATATCCACTCACCGCAAGTTACTGTCAAAGAGTCTCTC
ATCTACTCTGCTTTCCTTCGTCTCCCAAAAGAAGTCACTAAATATGAGAAAATGGTAACA
CTTCTTCTTGTAACTTAACACCATTTATAATAGGATCTCAATCCATACACCAACTTTTGC
AGAGATTTGTGGATGAAGTGATGGAGCTAGTAGAGCTAGAGAGTCTAAAAGACGCGGTGG
TGGGGCTTCCAGGTATCACAGGGTTATCAACAGAGCAGAGGAAGAGACTTACAATTGCAG
TGGAATTAGTGGCGAATCCATCTATAATCTTCATGGATGAGCCAACCTCTGGTCTTGACG
CTAGGGCTGCAGCGATTGTGATGAGGACAGTGAGGAATACAGTG
   
Sequence Name 66-K035535-022-012-B07C-KC14
GenBank Accession LN511542 [GenBank]
Sequencing primer gene specific primer, KC14: 5'-TCCTGTGTCAACTGTATTCCTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K035535-022-012-B07C-KC14
TAGCGTCAGACCAGAGGTTGGCTCATCCATGAAGATTATAGATGGATTCGCCACTAATTC
CACTGCAATTGTAAGTCTCTTCCTCTGCTCTGTTGATAACCCTGTGATACCTGGAAGCCC
CACCACCGCGTCTTTTAGACTCTCTAGCTCTACTAGCTCCATCACTTCATCCACAAATCT
CTGCAAAAGTTGGTGTATGGATTGAGATCCTATTATAAATGGTGTTAAGTTACAAGAAGA
AGTGTTACCATTTTCTCATATTTAGTGACTTCTTTTGGGAGACGAAGGAAAGCAGAGTAG
ATGAGAGACTCTTTGACAGTAACTTGCGGTGAGTGGATATCGTTCTGTTCACAATAACCA
GAGATTCTTGCAAAGGTTTCTTGTCTTTTAGGGAAACCAGAGATTCTGATGTCTCCTTCG
ATGTAACCACCGGTTTTTCTACCAGCTAAAACATCCATTAAAGTGGTCTTACCAGCTCCA
CTAACTCCCATCAAAGCTGTTAATACCCCAAATGTAAATGGCTATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACTAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11