Sequences from confirmation amplicon


Confirmation sequences for line 012H03 and hit At1g25270 are shown.
Line ID 012H03
Locus At1g25270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8859736
Primers used for PCR 02cp - 3144, show primer details
   
Sequence Name 58-K108745-0022-012-H03C-3144
GenBank Accession LN509727 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K108745-0022-012-H03C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGACGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTTCC
CGGACTTGCAGTATAATCGGTGCCATGATAATGGTGGGAGGAGTATACCTAGTGGTATGG
TGTAAAATGAAGGAAAAAAAGAGTGCGTCTACGACTTCGGACCATATTGAAACAAATAAG
AATAACAAAGAACTGGACCTTGGCAATCTTTCATCAGTAAATAGAGATGTCCCCTGAATC
CCCATATGGGTTACATGATCAAATATAGTTTGAGTCTACCATAGAAGCAGAATATAAAAT
AGGGTCGGTAAGTGAATTAAGATCCAGCTGTATGCCTCAATTGGTGTTAAGTAGCCTTTG
AAGTATATCTTTTTCAATTTTTAACGTTACTTCAATATTAAAACACAATTACAACTTGCT
ACTTAATTTTACCTCTAAAATGCTATTTTGTTATTGTCTAAGTGACTAAGTGGTAAATTT
GTTGTAAAAAAATTATGACCGCCACAACATTTCATCTCACATTTTATAGTTTTGAAGTGA
TTAATTCATCTTCACATATGTGGTTTTCATTTACCGTTTATTACTCTCTTTTTTTGGCTC
GAGGTTTGGAACTCAACAAAATGT
   
Sequence Name 74-K108746-0022-012-H03C-02cp
GenBank Accession LN513091 [GenBank]
Sequencing primer gene specific primer, 02cp: 5'-AAACATTTTGTTGAGTTCCAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K108746-0022-012-H03C-02cp
ACGGTAATGAAACCACATATGTGAAGATGAATTAATCACTTCAAAACTATAAAACGTGAG
ATGAAATGTTGTGGCGGTCATAATTTTTTTACAACAAATTTACCACTTAGTCACTTAGAC
AATAACAAAATAGCATTTTAGAGGTAAAATTAAGTAGCAAGTTGTAATTGTGTTTTAATA
TTGAAGTAACGTTAAAAATTGAAAAAGATATACTTCAAAGGCTACTTAACACCAATTGAG
GCATACAGCTGGATCTTAATTCACTTACCGACCCTATTTTATATTCTGCTTCTATGGTAG
ACTCAAACTATATTTGATCATGTAACCCATATGGGGATTCAGGGGACATCTCTATTTACT
GATGAAAGATTGCCAAGGTCCAGTTCTTTGTTATTCTTATTTGTTTCAATATGGTCCGAA
GTCGTAGACGCACTCTTTTTTTCCTTCATTTTACACCATACCACTAGGTATACTCCTCCC
ACCATTATCATGGCACCGATTATACTGCAAGTCCGGGAAAACACTGATAGTTTAAACCGA
AGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTC
CTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAG
TGGAGATATCACATCAATCC

Last Updated on 06.12.2018 16:11