Sequences from confirmation amplicon


Confirmation sequences for line 012H08 and hit At4g19700 are shown.
Line ID 012H08
Locus At4g19700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10714726
Primers used for PCR IM09 - 8474, show primer details
   
Sequence Name 42-K032522-022-012-H08C-IM09
GenBank Accession LN504357 [GenBank]
Sequencing primer gene specific primer, IM09: 5'-ACGAACCTATCGATCTCTGACTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K032522-022-012-H08C-IM09
TTATCAACGACGGTGATGATGAGTCAACGCTACGGACCTCCGTTTAACCGGAGCGTTTGA
GTCTGAGAATTGAAAATCTCGCTGGCGTTTTGTAGACAGAGGAGGAACAGTGAAATTGTA
ACTGAGACCACTTTCTGCTTTGTTGAAGTTGGCTTTCGCAGCTAAAGGCTCAACGGCGAA
AGATTCTCCGGTTATTAACGGAACTTCACCGCCGGTAAACTCAAACTCGCCGTGGTTCAT
GTTTTCTTGAAATTTAACACAATCTCTGCAAATAACAAAAATAAAAAAGATTAGAGGAAA
ATAAAAACAGAGGAAGTTTGTTTGTGAGAAGAATATAAAAATGAGATTTTTACCTGTTTG
GTGATATAAACTGAGAGAAAATGTTCATGTGATGAGCTTGAACAGCCATTGTTGTTGAAA
AAAGCTCCTAGAGATTCTGAAACAAAGAGAGAGAGAAGAGGGAGGAGTTGTGGCGACTGG
TGAAGAAATCTATGGGATTGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 42-K032522-022-012-H08C-8409
GenBank Accession LN504356 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K032522-022-012-H08C-8409
TCGGACTGAGCCTTTACAATTGAATAATCCTGATCAATCCCATAGATTTCTTCACCAGTC
GCCACAACTCCTCCCTCTTCTCTCTCTCTTTGTTTCAGAATCTCTAGGAGCTTTTTTCAA
CAACAATGGCTGTTCAAGCTCATCACATGAACATTTTCTCTCAGTTTATATCACCAAACA
GGTAAAAATCTCATTTTTATATTCTTCTCACAAACAAACTTCCTCTGTTTTTATTTTCCT
CTAATCTTTTTTATTTTTGTTATTTGCAGAGATTGTGTTAAATTTCAAGAAAACATGAAC
CACGGCGAGTTTGAGTTTACCGGCGGTGAAGTTCCGTTAATAACCGGAGAATCTTTCGCC
GTTGAGCCTTTAGCTGCGAAAGCCAACTTCAACAAAGCAGAAAGTGGTCTCAGTTACAAT
TTCACTGTTCCTCCTCTGTCTACAAAACGCCAGCGAGATTTTCAATTCTCAGACTCAAAC
GCTCCGGTTAAACGGAGGTCCGTAGCGTTTGACTCATCATCACCGTCGTTGATAAACGTT
GAACTCGTATCTCAGATTCAAAACCAACAACAGTCAGAGA

Last Updated on 06.12.2018 16:11