Sequences from confirmation amplicon


Confirmation sequences for line 014A07 and hit At3g06820 are shown.
Line ID 014A07
Locus At3g06820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2151355
Primers used for PCR KU42 - 8474, show primer details
   
Sequence Name 55-K113261-0022-014-A07C-KU42
GenBank Accession LN508869 [GenBank]
Sequencing primer gene specific primer, KU42: 5'-GAAATATACACAAAGAAGACGGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K113261-0022-014-A07C-KU42
GATCCTCTTCTGCTCGGCTGCCAGAACCGTGAACCAGATGAGATGGTCTCCCGTTTGTCT
CTGCTCCTTTAAGTTTTTGTGCCTCCAAAACCTCAGCTTCATCCATCAGCATTGCTAACT
GCACAACACACAACAGGTTTTAGACCCAACCAAAAAAAAGCATCATCCATGAGAGAGCTT
TATTTATGAGGAGACACATACCCGGATCTTGTTCTCCTTCTGCCTATCTTGAAGTGCGTT
AATGGCAGGACTCAAGCTGCAAAAGCATGAAGTTCATATAACGAATGACAAAACCACGTA
TCAAATGTTTTAACACGCTTCACTGGTTAGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 36-K113260-0022-014-A07C-8409
GenBank Accession LN502295 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K113260-0022-014-A07C-8409
CCAGTGAAGCGTGTTAAAACATTTGATACGTGGTTTTGTCATTCGTTATATGAACTTCAT
GCTTTTGCAGCTTGAGTCCTGCCATTAACGCACTTCAAGATAGGCAGAAGGAGAACAAGA
TCCGGGTATGTGTCTCCTCATAAATAAAGCTCTCTCATGGATGATGCTTTTTTTTGGTTG
GGTCTAAAACCTGTTGTGTGTTGTGCAGTTAGCAATGCTGATGGATGAAGCTGAGGTTTT
GGAGGCACAAAAACTTAAAGGAGCAGAGACAAGCCGGAGACCATCTCATCTGGTTCACGG
TTCTGGCAGCCGAAGCAGAAGAGGATCGTAGAAAGACCTGCAACTAAAGACACATGCCCG
TCTTCTTTGTGTATATTT

Last Updated on 06.12.2018 16:11