Sequences from confirmation amplicon


Confirmation sequences for line 014D07 and hit At2g41140 are shown.
Line ID 014D07
Locus At2g41140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17151970
Primers used for PCR NE27 - 8474, show primer details
   
Sequence Name 51-K102433-0022-014-D07C-8409
GenBank Accession LN507458 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K102433-0022-014-D07C-8409
TGCAAACCCGTCAAATTTTCTATTTAACATGAAGATGAGTTTGATATGAGAAATCTTGAG
TCAATTTCAACATCTCTAACCTGGCTTGACATAGTCAGAAAGACCAAAATCAATTGCTTT
CAGAGGAGAAGTCTCATCCTTGGTAGAGAAAAGAAAGTTCTGTGAAAGGTAAACAAACAA
ATAGAAAGAAACATAACCGTCATTATCACTCTGCATAAGTTAGTGGAACTCATTTCTATG
TGTACAAAGACTGCTTAATGTGATGGGACCTAGAAAGTGAAGTGTCAACAAAAGCCAAAA
TGATTTACAAAAAAAAACAATGAGAGAACAATCTAGAAATTAAACATCACCATGGATTAT
AAAGTACCTCGGGTTTAAGGTCACGGTGAACCACACCTTGTAGATGACAGTAAGCCACAA
CACTGAG
   
Sequence Name 51-K102434-0022-014-D07C-NE27
GenBank Accession LN507459 [GenBank]
Sequencing primer gene specific primer, NE27: 5'-ATGGTTCAGATTCTCAGTGTTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K102434-0022-014-D07C-NE27
ACCTTAACCCGAGGTACTTTATAATCCATGGTGATGTTTAATTTCTAGATTGTTCTCTCA
TTGTTTTTTTTTGTAAATCATTTTGGCTTTTGTTGACACTTCACTTTCTAGGTCCCATCA
CATTAAGCAGTCTTTGTACACATAGAAATGAGTTCCACTAACTTATGCAGAGTGATAATG
ACGGTTATGTTTCTTTCTATTTGTTTGTTTACCTTTCACAGAACTTTCTTTTCTCTACCA
AGGATGAGACTTCTCCTCTGAAAGCAATTGATTTTGGTCTTTCTGACTATGTCAAGCCAG
GTTAGAGATGTTGAAATTGACTCAAGATTTCTCATATCAAACTCATCTTCATGTTAAATA
GAAAATTTGACGGTGTTTTGCAATTCTACATGTCCGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11